OncoTargets and Therapy (Jan 2019)
Clinical significance of miR-195 in hepatocellular carcinoma and its biological function in tumor progression
Abstract
Xiaoyan Chen,1 Angang Wang2 1Department of Laboratory Medicine, Women and Children’s Hospital of Linyi City, Shandong 276000, People’s Republic of China; 2Department of Laboratory Medicine, People’s Hospital of Yutai County, Shandong 272300, People’s Republic of China Background: Hepatocellular carcinoma (HCC) is one of the most lethal cancer types all over the world. Chronic viral hepatitis B and hepatitis C are risk factors that are associated with the development of HCC. The aim of this study is to identify the diagnostic role of serum miR-195 in HCC.Patients and methods: The expression levels of miR-195 were detected in 120 HCC patients, 64 hepatitis only patients, and 118 healthy control as well as 4 HCC cell lines, by using quantitative real-time PCR. The association of miR-195 with clinicopathological parameters of patients was analyzed with the chi-squared test. The receiver operating characteristic (ROC) curve was adopted to estimate the potential diagnostic value of miR-195. The cell experiments were carried out to verify the functional role of miR-195.Results: The expression of miR-195 was downregulated in HCC cells and serum of patients compared to the controls (all P<0.05). The miR-195 expression was associated with lymph node metastasis and TNM stage. The ROC curve analysis showed that miR-195 may be a noninvasive diagnostic marker for patients. By using miR-195 mimic or inhibitor, cell proliferation, migration, and invasion were inhibited by miR-195 overexpression but promoted by reduced expression of miR-195.Conclusion: The downregulation of miR-195 may serve as a novel diagnostic biomarker for differentiating HCC patients, healthy individuals, and hepatitis patients, and may involve in the tumor progression of HCC. Keywords: miRNA-195, diagnosis, proliferation, migration, invasion, hepatocellular carcinoma
