Myeloperoxidase enhances the migration and invasion of human choriocarcinoma JEG-3 cells
Z.N. Mihalic,
T. Kloimböck,
N. Cosic-Mujkanovic,
P. Valadez-Cosmes,
K. Maitz,
O. Kindler,
C. Wadsack,
A. Heinemann,
G. Marsche,
M. Gauster,
J. Pollheimer,
J. Kargl
Affiliations
Z.N. Mihalic
Otto Loewi Research Center, Division of Pharmacology, Medical University of Graz, Austria
T. Kloimböck
Otto Loewi Research Center, Division of Pharmacology, Medical University of Graz, Austria
N. Cosic-Mujkanovic
Otto Loewi Research Center, Division of Pharmacology, Medical University of Graz, Austria
P. Valadez-Cosmes
Otto Loewi Research Center, Division of Pharmacology, Medical University of Graz, Austria
K. Maitz
Otto Loewi Research Center, Division of Pharmacology, Medical University of Graz, Austria
O. Kindler
Otto Loewi Research Center, Division of Pharmacology, Medical University of Graz, Austria
C. Wadsack
Department of Obstetrics and Gynecology, Medical University of Graz, Austria; BioTechMed-Graz, 8010, Graz, Austria
A. Heinemann
Otto Loewi Research Center, Division of Pharmacology, Medical University of Graz, Austria; BioTechMed-Graz, 8010, Graz, Austria
G. Marsche
Otto Loewi Research Center, Division of Pharmacology, Medical University of Graz, Austria; BioTechMed-Graz, 8010, Graz, Austria
M. Gauster
Division of Cell Biology, Histology and Embryology, Medical University of Graz, Austria
J. Pollheimer
Department of Obstetrics and Gynecology, Reproductive Biology Unit, Maternal-Fetal Immunology Group, Medical University of Vienna, Austria
J. Kargl
Otto Loewi Research Center, Division of Pharmacology, Medical University of Graz, Austria; BioTechMed-Graz, 8010, Graz, Austria; Corresponding author. Otto Loewi Research Center, Division of Pharmacology, Medical University of Graz, Austria.
Myeloperoxidase (MPO) is one of the most abundant proteins in neutrophil granules. It catalyzes the production of reactive oxygen species, which are important in inflammation and immune defense. MPO also binds to several proteins, lipids, and DNA to alter their function. MPO is present at the feto-maternal interface during pregnancy, where neutrophils are abundant. In this study, we determined the effect of MPO on JEG-3 human choriocarcinoma cells as a model of extravillous trophoblasts (EVTs) during early pregnancy. We found that MPO was internalized by JEG-3 cells and localized to the cytoplasm and nuclei. MPO internalization and activity enhanced JEG-3 cell migration and invasion, whereas this effect was impaired by pre-treating cells with heparin, to block cellular uptake, and MPO-activity inhibitor 4-ABAH. This study identifies a novel mechanism for the effect of MPO on EVT function during normal pregnancy and suggests a potential role of MPO in abnormal pregnancies.
