Nature Communications (Mar 2020)
Virtual-freezing fluorescence imaging flow cytometry
- Hideharu Mikami,
- Makoto Kawaguchi,
- Chun-Jung Huang,
- Hiroki Matsumura,
- Takeaki Sugimura,
- Kangrui Huang,
- Cheng Lei,
- Shunnosuke Ueno,
- Taichi Miura,
- Takuro Ito,
- Kazumichi Nagasawa,
- Takanori Maeno,
- Hiroshi Watarai,
- Mai Yamagishi,
- Sotaro Uemura,
- Shinsuke Ohnuki,
- Yoshikazu Ohya,
- Hiromi Kurokawa,
- Satoshi Matsusaka,
- Chia-Wei Sun,
- Yasuyuki Ozeki,
- Keisuke Goda
Affiliations
- Hideharu Mikami
- Department of Chemistry, The University of Tokyo
- Makoto Kawaguchi
- Department of Chemistry, The University of Tokyo
- Chun-Jung Huang
- Department of Chemistry, The University of Tokyo
- Hiroki Matsumura
- Department of Chemistry, The University of Tokyo
- Takeaki Sugimura
- Department of Chemistry, The University of Tokyo
- Kangrui Huang
- Department of Chemistry, The University of Tokyo
- Cheng Lei
- Department of Chemistry, The University of Tokyo
- Shunnosuke Ueno
- Department of Chemistry, The University of Tokyo
- Taichi Miura
- Department of Chemistry, The University of Tokyo
- Takuro Ito
- Department of Chemistry, The University of Tokyo
- Kazumichi Nagasawa
- Center for Stem Cell Biology and Regenerative Medicine, The University of Tokyo
- Takanori Maeno
- Center for Stem Cell Biology and Regenerative Medicine, The University of Tokyo
- Hiroshi Watarai
- Center for Stem Cell Biology and Regenerative Medicine, The University of Tokyo
- Mai Yamagishi
- Department of Biological Sciences, The University of Tokyo
- Sotaro Uemura
- Department of Biological Sciences, The University of Tokyo
- Shinsuke Ohnuki
- Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo
- Yoshikazu Ohya
- Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo
- Hiromi Kurokawa
- Department of Clinical Research and Regional Innovation, Faculty of Medicine, University of Tsukuba
- Satoshi Matsusaka
- Department of Clinical Research and Regional Innovation, Faculty of Medicine, University of Tsukuba
- Chia-Wei Sun
- Department of Photonics, National Chiao Tung University
- Yasuyuki Ozeki
- Department of Electrical Engineering and Information Systems, The University of Tokyo
- Keisuke Goda
- Department of Chemistry, The University of Tokyo
- DOI
- https://doi.org/10.1038/s41467-020-14929-2
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 11
Abstract
High throughput imaging flow cytometry suffers from trade-offs between throughput, sensitivity and spatial resolution. Here the authors introduce a method to virtually freeze cells in the image acquisition window to enable 1000 times longer signal integration time and improve signal-to-noise ratio.
