Journal of Translational Medicine (Jan 2011)

Myocardium-derived conditioned medium improves left ventricular function in rodent acute myocardial infarction

  • Yeh Kuo-Ho,
  • Chua Sarah,
  • Chang Li-Teh,
  • Tsai Tzu-Hsien,
  • Lin Yu-Chun,
  • Sun Cheuk-Kwan,
  • Kao Ying-Hsien,
  • Leu Steve,
  • Wu Chiung-Jen,
  • Fu Morgan,
  • Yip Hon-Kan

DOI
https://doi.org/10.1186/1479-5876-9-11
Journal volume & issue
Vol. 9, no. 1
p. 11

Abstract

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Abstract Background We investigated whether myocardium-derived conditioned medium (MDCM) is effective in preserving left ventricular (LV) function in a rat acute myocardial infarction (AMI) model. Methods Adult male Sprague-Dawley (SD) rats (n = 36) randomized to receive either left coronary artery ligation (AMI induction) or thoracotomy only (sham procedure) were grouped as follows (n = 6 per group): Group I, II, and III were sham-controls treated by fresh medium, normal rat MDCM, and infarct-related MDCM, respectively. Group IV, V, and VI were AMI rats treated by fresh medium, normal MDCM, and infarct-related MDCM, respectively. Either 75 μL MDCM or fresh medium was administered into infarct myocardium, followed by intravenous injection (3 mL) at postoperative 1, 12, and 24 h. Results In vitro studies showed higher phosphorylated MMP-2 and MMP-9, but lower α-smooth muscle actin and collagen expressions in neonatal cardiac fibroblasts treated with MDCM compared with those in the cardiac fibroblasts treated with fresh medium (all p Conclusion MDCM therapy reduced cardiac fibrosis and oxidative stress, enhanced angiogenesis, and preserved 90-day LV function in a rat AMI model.