مجله بیوتکنولوژی کشاورزی (Dec 2021)
Study on the genetic diversity of tomato’s cultivars via SCoT marker
Abstract
Objective This study was conducted to investigate the application of the start codon targeted (SCoT) markers in the genetic diversity of tomato. In addition, the genetic diversity in important cultivars of tomato, which have been previously cultivated, are being cultivated, or are hoped to be cultivated in Iran were assessed. Materials and Methods Ninety nine 99 tomato cultivars were investigated for SCoT polymorphism. DNA isolation and SCoT analysis were carried out using the fresh leaf samples. Thirty-six SCoT primers were initially screened for analysis and fifteen primers were considered for the further analysis. Results The cultivars produced 207 amplicons while the 206 were polymorphic. Amplicon size varied from 250 to 3200 base pair (bp). The average of polymorphism and the mean of polymorphic information content were 99.52 and 0.30, respectively. Also, Jaccard's similarity coefficient was applied and the mean Jaccard genetic similarity coefficient was 0.52. According to Jaccard's coefficient, the lowest similarity (0.17) has been observed for cultivars 34 and 97 while the highest similarity (0.84) were detected between cultivars 86 and 87. In addition, the cluster analysis was conducted based on Jaccard similarity coefficient and centroid method which classified cultivars into three clusters. Besides, the principal coordinate analysis was considered. Accordingly, the cultivars divided into four groups and the first three components explained 58.82% of the molecular variation. The results of the principal coordinate analysis were largely consistent with the results of the cluster analysis. Having high polymorphic information content, marker index, effective multiplex ratio, and resolution power, SCoT12 and SCoT23 primers were properly effective in differentiating the cultivars. Conclusions This study showed that SCoT molecular markers are appropriate for investigating the genetic diversity amongst tomato genotypes and generate a high level of polymorphism. Thus, these markers have considerable efficiency in differentiating tomato genotypes. In addition, this investigation indicated that SCoT12 and SCoT23 primers were suitably effective in differentiating the cultivars. Furthermore, our resech claimed that tomato cultivars that are used in Iran do not have high genetic diversity while for more precise conclusion; it is suggested to practice more SCoT primers along with other markers.
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