The influence of the preparation “Bendamin” on the morphological and biochemical indices of blood of rats in experimental modeling of heart failure

Abstract

The purpose of this work was to study the influence of “Bendamin” on the morphological and biochemical indices of rat blood in experimental modeling of heart failure. The investigation were performed on white sexually mature young male-rats of Wistar line weighing 180–200 g, kept at the standard ration of the institute vivarium of the State Research Institute of Veterinary Preparations and Fodder Additives. To create a model of doxorubicin-induced cardiomyopathy, 24 male rats were selected. Animals were divided into 3 groups of 6 animals in each: control group – intact animals; experimental group E1, in which animals were modeled doxorubicin-induced cardiomyopathy by intraperitoneal injected of doxorubicin at a dose of 2.5 mg/kg 3 times a week for two weeks; experimental group E2, in which animals, after injection of doxorubicin, the preparation “Bendamin” was injected in the dose 20 mg/kg intragastrically. For experimental modeling of heart a decrease in the number of red blood cells was found in the blood of rats of the first experimental group by 34.7 % and hemoglobin level – by 24.2 %, with a simultaneous increase in the average hemoglobin content in one erythrocyte by 16.2 %. Doxorubicin-induced cardiomyopathy in rats of the first experimental group was accompanied by a decrease in leukocyte count by 22.2 %, an increase in uric acid level by almost in 2 times, creatinine by 11.4 %, cholesterol by 61.1 %. In the research of the protein synthesizing function of the liver of rats in experimental modeling of heart failure, a decrease in total protein by 9.6 % was found. These changes were accompanied by a slight decrease in albumin levels and a slight increase in globulin levels. By intoxication with doxorubicin, rats of the first experimental group showed a violation of the functional state of the liver, indicating an increase in the activity of aminotransferases in their blood, namely: AlAT by 34.2 %, AsAT – by 21.7 %, accordingly. Positive action of cardio preparation “Bendamin” on rats organism under conditions of creation of a model of doxorubicin-induced cardiomyopathy was installed, manifested by the normalization of hematological and biochemical parameters, functional status and protein synthesis of the liver.

Keywords

• pharmacology, “bendamin” preparation, rats, heart failure, cardiomyopathy