PpMAPK6 regulates peach bud endodormancy release through interactions with PpDAM6

Yu-zheng ZHANG; Chen XU; Wen-li LU; Xiao-zhe WANG; Ning WANG; Xiang-guang MENG; Yu-hui FANG; Qiu-ping TAN; Xiu-de CHEN; Xi-ling FU; Ling LI

Journal of Integrative Agriculture (Jan 2023)

PpMAPK6 regulates peach bud endodormancy release through interactions with PpDAM6

Yu-zheng ZHANG,
Chen XU,
Wen-li LU,
Xiao-zhe WANG,
Ning WANG,
Xiang-guang MENG,
Yu-hui FANG,
Qiu-ping TAN,
Xiu-de CHEN,
Xi-ling FU,
Ling LI

Affiliations

Yu-zheng ZHANG: College of Horticultural Science and Engineering, Shandong Agricultural University, Tai’an 271018, P.R.China; State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, P.R.China; Shandong Collaborative Innovation Center for Fruit & Vegetable Production with High Quality and Efficiency, Tai’an 271018, P.R.China
Chen XU: College of Horticultural Science and Engineering, Shandong Agricultural University, Tai’an 271018, P.R.China; State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, P.R.China; Shandong Collaborative Innovation Center for Fruit & Vegetable Production with High Quality and Efficiency, Tai’an 271018, P.R.China
Wen-li LU: College of Horticultural Science and Engineering, Shandong Agricultural University, Tai’an 271018, P.R.China; State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, P.R.China; Shandong Collaborative Innovation Center for Fruit & Vegetable Production with High Quality and Efficiency, Tai’an 271018, P.R.China
Xiao-zhe WANG: College of Horticultural Science and Engineering, Shandong Agricultural University, Tai’an 271018, P.R.China; State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, P.R.China; Shandong Collaborative Innovation Center for Fruit & Vegetable Production with High Quality and Efficiency, Tai’an 271018, P.R.China
Ning WANG: College of Horticultural Science and Engineering, Shandong Agricultural University, Tai’an 271018, P.R.China; State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, P.R.China; Shandong Collaborative Innovation Center for Fruit & Vegetable Production with High Quality and Efficiency, Tai’an 271018, P.R.China
Xiang-guang MENG: College of Horticultural Science and Engineering, Shandong Agricultural University, Tai’an 271018, P.R.China; State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, P.R.China; Shandong Collaborative Innovation Center for Fruit & Vegetable Production with High Quality and Efficiency, Tai’an 271018, P.R.China
Yu-hui FANG: College of Horticultural Science and Engineering, Shandong Agricultural University, Tai’an 271018, P.R.China; State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, P.R.China; Shandong Collaborative Innovation Center for Fruit & Vegetable Production with High Quality and Efficiency, Tai’an 271018, P.R.China
Qiu-ping TAN: College of Horticultural Science and Engineering, Shandong Agricultural University, Tai’an 271018, P.R.China; State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, P.R.China; Shandong Collaborative Innovation Center for Fruit & Vegetable Production with High Quality and Efficiency, Tai’an 271018, P.R.China
Xiu-de CHEN: College of Horticultural Science and Engineering, Shandong Agricultural University, Tai’an 271018, P.R.China; State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, P.R.China; Shandong Collaborative Innovation Center for Fruit & Vegetable Production with High Quality and Efficiency, Tai’an 271018, P.R.China
Xi-ling FU: College of Horticultural Science and Engineering, Shandong Agricultural University, Tai’an 271018, P.R.China; State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, P.R.China; Shandong Collaborative Innovation Center for Fruit & Vegetable Production with High Quality and Efficiency, Tai’an 271018, P.R.China; Correspondence FU Xi-ling
Ling LI: College of Horticultural Science and Engineering, Shandong Agricultural University, Tai’an 271018, P.R.China; State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, P.R.China; Shandong Collaborative Innovation Center for Fruit & Vegetable Production with High Quality and Efficiency, Tai’an 271018, P.R.China; Correspondence LI Ling

Journal volume & issue: Vol. 22, no. 1
pp. 139 – 148

Abstract

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The MADS-box (DAM) gene PpDAM6, which is related to dormancy, plays a key role in bud endodormancy release, and the expression of PpDAM6 decreases during endodormancy release. However, the interaction network that governs its regulation of the endodormancy release of flower buds in peach remains unclear. In this study, we used yeast two-hybrid (Y2H) assays to identify a mitogen-activated protein kinase, PpMAPK6, that interacts with PpDAM6 in a peach dormancy-associated SSHcDNA library. PpMAPK6 is primarily located in the nucleus, and Y2H and bimolecular fluorescence complementation (BiFC) assays verified that PpMAPK6 interacts with PpDAM6 by binding to the MADS-box domain of PpDAM6. Quantitative real-time PCR (qRT-PCR) analysis showed that the expression of PpMAPK6 was opposite that of PpDAM6 in the endodormancy release of three cultivars with different chilling requirements (Prunus persica ‘Chunjie’, Prunus persica var. nectarina ‘Zhongyou 5’, Prunus persica ‘Qingzhou peach’). In addition, abscisic acid (ABA) inhibited the expression of PpMAPK6 and promoted the expression of PpDAM6 in flower buds. The results indicated that PpMAPK6 might phosphorylate PpDAM6 to accelerate its degradation by interacting with PpDAM6. The expression of PpMAPK6 increased with decreasing ABA content during endodormancy release in peach flower buds, which in turn decreased the expression of PpDAM6 and promoted endodormancy release.

Published in Journal of Integrative Agriculture

ISSN: 2095-3119 (Print); 2352-3425 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Agriculture (General)
Website: https://www.journals.elsevier.com/journal-of-integrative-agriculture

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