International Journal of Nanomedicine (Nov 2019)
Regulating Preparation Of Functional Alginate-Chitosan Three-Dimensional Scaffold For Skin Tissue Engineering
Abstract
Tonghe Zhu,1 Jia Jiang,1 Jinzhong Zhao,1 Sihao Chen,2,3 Xiaoyu Yan1 1Department of Sports Medicine, Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, People’s Republic of China; 2Scientific Research Department of Shanghai University of Engineering Science, Shanghai 201620, People’s Republic of China; 3Multidisciplinary Center for Advanced Materials of Shanghai University of Engineering Science, Shanghai University of Engineering Science, Shanghai 201620, People’s Republic of ChinaCorrespondence: Xiaoyu YanDepartment of Sports Medicine, Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, No. 600 Yishan Road, Shanghai 200233, People’s Republic of ChinaTel/Fax +86 21 24056088Email [email protected]: In this study, we attempted to regulate the preparation of Alg-CS-Flu three-dimensional scaffolds via a facile freeze-drying method combined with amidation.Materials and methods: Three-dimensional porous flurbiprofen-grafted alginate (Alg)–chitosan (CS) scaffolds were successfully prepared by a facile freeze-drying method combined with amidation for skin tissue engineering applications. Alg-CS composite was first used to load flurbiprofen (Flu), which is a kind of anti-inflammatory non-steroidal molecule. The Flu-loaded Alg/CS composite solution, through freeze-drying and 1-ethyl-3(3-(dimethylamino)propyl) carbodiimide/N-hydroxysuccinimide crosslinking to form an Alg-CS-Flu scaffold, exhibited a uniform and porous morphology that was characterized using scanning electron microscopy. The Alg-CS-Flu as-prepared scaffold was also characterized using Fourier-transform infrared spectroscopy, water contact angle, thermal properties, and stress-strain testing.Results: The results reveal that Flu was successfully grafted onto the surfaces of the Alg-CS-Flu scaffold, which showed good hydrophilicity and appropriate mechanical properties. Furthermore, cell viability, cell morphology from cells cultured in vitro, and hematoxylin-eosin staining after the graft was subcutaneously embedded in mice for 7 d demonstrated that the Alg-CS-Flu scaffold had no unfavorable effects on the adhesion and proliferation of fibroblasts, as well as a good anti-inflammatory property.Conclusion: The developed Alg-CS-Flu scaffold is proposed as a promising material or skin tissue engineering application.Keywords: three-dimensional scaffold, flurbiprofen, freeze-drying, amidation, skin tissue engineering
