Protective effect of Bone marrow mesenchymal cells on Testis Histologic and Histolomorphometric properties in Mice following Spermatic cord Toesion-Detorsion

Abstract

Background & Aim: According to the disturbance in testis circulation and subsequently the anemia of an organ, it can disrupt fertility. In this study, the protective effect of mesenchymal gastric lavage cells on testicular histomorphometry after the torsion of Tursion-Detursion (T-D) of the testicular band was investigated in a comparative manner. Methods: In this experimental study, 30 adult mice were randomly divided into three equal groups; the control group was Sham, in this group only the abdominal cavity was opened and then closed. Torsion Torsion Group (T-D), in this group, the fracture torsion was performed in the left testicular fracture. After the circulating blood was restored to the rat's ellipse, a phosphate buffer of 10 μL was injected. Mesenchymal cell group (T-D + Msc), after torsion of the defect in mice, the mesenchymal cells were injected into half a million volumes, 10 microliters, of the testicular network. At the end of the treatment period (35 days), after sampling the testicular tissue of the left side and stabilizing the sample, paraffin sections were stained with Hematoxylin Eosin and Masonium trichrome (for cervical counts), then the tissue parameters were evaluated. The data were analyzed using ANOVA test and Tukey post hoc test. Results: Our results showed that mesenchymal treatment (T-D + MSc) had a significant increase in the mean spermatogenesis, active serotylated cells and leydig cells, diameter of the menisis tubes, epithelium thickness and testis capsule compared with the defect torsion group (TD) P<0.05). In the study of the number of arteries in the T-D group, there was a significant decrease in the T-D + MSc group (p<0.05). Conclusion: Using of Mesenchymal cells can improve the process of repairing the damage caused by torsion defect in the testicles by increasing angiogenesis and tissue repair.

Keywords

• Microsurgery
• Testis
• Testicular Defect Torsion
• Mesenchymal Cell