Nature Communications (Oct 2017)

Algorithm for post-clustering curation of DNA amplicon data yields reliable biodiversity estimates

  • Tobias Guldberg Frøslev,
  • Rasmus Kjøller,
  • Hans Henrik Bruun,
  • Rasmus Ejrnæs,
  • Ane Kirstine Brunbjerg,
  • Carlotta Pietroni,
  • Anders Johannes Hansen

DOI
https://doi.org/10.1038/s41467-017-01312-x
Journal volume & issue
Vol. 8, no. 1
pp. 1 – 11

Abstract

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A central problem in biodiversity estimation from genetic markers is the ability of algorithms to retain ‘true’ species while discarding artefacts. Here, the authors present a new post-clusturing curation algorithm using OTU co-occurrences to estimate plant biodiversity from soil samples.