Biological Procedures Online (Jul 2011)

A rapid and easy method for the DNA extraction from <it>Cryptococcus neoformans</it>

  • Sellami Hayet,
  • Sellami Amira,
  • Jarboui Mohammed,
  • Mseddi Fatma,
  • Ayadi Ali

DOI
https://doi.org/10.1186/1480-9222-13-5
Journal volume & issue
Vol. 13, no. 1
p. 5

Abstract

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Abstract DNA isolation from C. neoformans is difficult due to a thick and resistant capsule. We have optimized a new and rapid DNA isolation method for Cryptococcus using a short urea treatment followed by a rapid method using a chelex resin suspension. This procedure is simpler than previously reported methods.