Scientia Agricola (Oct 2020)

Genetic diversity and heterotic grouping of sorghum lines using SNP markers

  • Karla Jorge da Silva,
  • Maria Marta Pastina,
  • Claudia Teixeira Guimarães,
  • Jurandir Vieira Magalhães,
  • Leonardo Duarte Pimentel,
  • Robert Eugene Schaffert,
  • Marcos de Oliveira Pinto,
  • Vander Fillipe de Souza,
  • Karine da Costa Bernardino,
  • Michele Jorge da Silva,
  • Aluízio Borém,
  • Cicero Beserra de Menezes

DOI
https://doi.org/10.1590/1678-992x-2020-0039
Journal volume & issue
Vol. 78, no. 6

Abstract

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ABSTRACT: Sorghum breeding programs are based predominantly on developing homozygous lines to produce single cross hybrids, frequently with relatively narrow genetic bases. The adoption of complementary strategies, such as genetic diversity study, enables a broader vision of the genetic structure of the breeding germplasm. The purpose of this study was to evaluate the genetic diversity of sorghum breeding lines using structure analysis, principal components (PC) and clustering analyses. A total of 160 sorghum lines were genotyped with 29,649 SNP markers generated by genotyping-by-sequencing (GBS). The PC and clustering analyses consistently divided the R (restorer) and B (maintainer) lines based on their pedigree, generating four groups. Thirty-two B and 21 R lines were used to generate 121 single-cross hybrids, whose performances were compared based on the diversity clustering of each parental line. The genetic divergence of B and R lines indicated a potential for increasing heterotic response in the development of hybrids. The genetic distance was correlated to heterosis, allowing for the use of markers to create heterotic groups in sorghum.

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