Development of Pectinase Based Nanocatalyst by Immobilization of Pectinase on Magnetic Iron Oxide Nanoparticles Using Glutaraldehyde as Crosslinking Agent
Tayyaba Behram,
Sidra Pervez,
Muhammad Asif Nawaz,
Shujaat Ahmad,
Amin Ullah Jan,
Haneef Ur Rehman,
Shahbaz Ahmad,
Nasir Mehmood Khan,
Farman Ali Khan
Affiliations
Tayyaba Behram
Department of Biotechnology, Shaheed Benazir Bhutto University, Sheringal Dir Upper 18000, Pakistan
Sidra Pervez
Department of Biochemistry, Shaheed Benazir Bhutto Women University, Peshawar 25000, Pakistan
Muhammad Asif Nawaz
Department of Biotechnology, Shaheed Benazir Bhutto University, Sheringal Dir Upper 18000, Pakistan
Shujaat Ahmad
Department of Pharmacy, Shaheed Benazir Bhutto University, Sheringal Dir Upper 18000, Pakistan
Amin Ullah Jan
Department of Biotechnology, Shaheed Benazir Bhutto University, Sheringal Dir Upper 18000, Pakistan
Haneef Ur Rehman
Department of Natural and Basic Sciences, University of Turbat, Kech, Turbat 92600, Pakistan
Shahbaz Ahmad
Department of Biological Science and Engineering, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China
Nasir Mehmood Khan
Department of Agriculture, Shaheed Benazir Bhutto University, Sheringal Dir Upper 18000, Pakistan
Farman Ali Khan
Department of Chemistry, Shaheed Benazir Bhutto University, Sheringal Dir Upper 18000, Pakistan
To increase its operational stability and ongoing reusability, B. subtilis pectinase was immobilized on iron oxide nanocarrier. Through co-precipitation, magnetic iron oxide nanoparticles were synthesized. Scanning electron microscopy (SEM) and energy dispersive electron microscopy (EDEX) were used to analyze the nanoparticles. Pectinase was immobilized using glutaraldehyde as a crosslinking agent on iron oxide nanocarrier. In comparison to free pectinase, immobilized pectinase demonstrated higher enzymatic activity at a variety of temperatures and pH levels. Immobilization also boosted pectinase’s catalytic stability. After 120 h of pre-incubation at 50 °C, immobilized pectinase maintained more than 90% of its initial activity due to the iron oxide nanocarrier, which improved the thermal stability of pectinase at various temperatures. Following 15 repetitions of enzymatic reactions, immobilized pectinase still exhibited 90% of its initial activity. According to the results, pectinase’s catalytic capabilities were enhanced by its immobilization on iron oxide nanocarrier, making it economically suitable for industrial use.
