Generation of three human induced pluripotent stem cell sublines (UCLAi005-A, UCLAi005-B and UCLAi005-C) for reproductive science research

Erica C. Pandolfi; Timothy J. Hunt; Sierra Goldsmith; Kellie Hurlbut; Sherman J. Silber; Amander T. Clark

Stem Cell Research (Jul 2021)

Generation of three human induced pluripotent stem cell sublines (UCLAi005-A, UCLAi005-B and UCLAi005-C) for reproductive science research

Erica C. Pandolfi,
Timothy J. Hunt,
Sierra Goldsmith,
Kellie Hurlbut,
Sherman J. Silber,
Amander T. Clark

Affiliations

Erica C. Pandolfi: Department of Molecular, Cell and Developmental Biology, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA USA; Molecular Biology Institute, University of California, Los Angeles, CA, USA
Timothy J. Hunt: Department of Molecular, Cell and Developmental Biology, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA USA; Molecular Biology Institute, University of California, Los Angeles, CA, USA
Sierra Goldsmith: Infertility Center of St. Louis, St. Luke's Hospital, St. Louis, MO, USA
Kellie Hurlbut: Infertility Center of St. Louis, St. Luke's Hospital, St. Louis, MO, USA
Sherman J. Silber: Infertility Center of St. Louis, St. Luke's Hospital, St. Louis, MO, USA
Amander T. Clark: Department of Molecular, Cell and Developmental Biology, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA USA; Molecular Biology Institute, University of California, Los Angeles, CA, USA; Corresponding author at: Department of Molecular, Cell and Developmental Biology, Los Angeles, CA 90095, USA.

Journal volume & issue: Vol. 54
p. 102409

Abstract

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We generated three human induced pluripotent stem cell (hiPSC) sublines from human dermal fibroblasts (HDF) (MZT05) generated from a skin biopsy donated from a previously fertile woman. The skin biopsy was broadly consented for generating hiPSC lines for biomedical research, including unique consent specifically for studying human fertility, infertility and germ cell differentiation. hiPSCs were reprogrammed using Sendai virus vectors and were subsequently positive for markers of self-renewal. Pluripotency was further verified using PluriTest analysis and in vitro differentiation was tested using Taqman Real-Time PCR assays. These sublines serve as controls for hiPSC research projects aimed at understanding the cell and molecular regulation of female fertility.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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