Genome sequencing of Enterococcus faecium NT04, an oral microbiota revealed the production of enterocin A/B active against oral pathogens
Nashwa Tarek,
Ahmed F. Azmy,
Ahmed S. Khairalla,
Medhat Abdel-Fattah,
Ohoud A. Jefri,
Mohamed Shaban,
Abdelaziz A.A. El-Sayed,
Ahmed O. El-Gendy
Affiliations
Nashwa Tarek
Department of Botany and Microbiology, Faculty of Science, Beni-Suef University, Egypt; Basic Science Department, Faculty of Oral and Dental Medicine, Nahda University Beni-Suef (NUB), Beni Suef, Egypt
Ahmed F. Azmy
Microbiology and Immunology Department, Faculty of Pharmacy, Beni-Suef University, Egypt
Ahmed S. Khairalla
Microbiology and Immunology Department, Faculty of Pharmacy, Beni-Suef University, Egypt; Department of Biology, University of Regina, 3737 Wascana Parkway, Regina, SK, S4S 0A2, Canada
Medhat Abdel-Fattah
Department of Botany and Microbiology, Faculty of Science, Beni-Suef University, Egypt
Ohoud A. Jefri
Department of Biological Science, Faculty of Science, King Abdulaziz University, Jeddah, 21589, Saudi Arabia
Mohamed Shaban
Department of Physics, Faculty of Science, Islamic University of Madinah, Al-Madinah Al-Munawarah, 42351, Saudi Arabia; Nanophotonics and Applications (NPA) Lab, Physics Department, Faculty of Science, Beni-Suef University, Beni-Suef, 62514, Egypt
Abdelaziz A.A. El-Sayed
Biology Department, Faculty of Science, Islamic University of Madinah, Al-Madinah Al- Munawarah, 42351, Saudi Arabia; Zoology Department, Faculty of Science, Zagazig University, Zagazig, 44519, Egypt; Corresponding author. Zoology Department, Faculty of Science, Zagazig University, Zagazig, 44519, Egypt.
Ahmed O. El-Gendy
Microbiology and Immunology Department, Faculty of Pharmacy, Beni-Suef University, Egypt
Objective: This study aimed to isolate and investigate a bacterium from an Egyptian adult's healthy oral cavity, focusing on its probiotic properties, especially its antagonistic activity against oral pathogens. Methods: The isolated bacterium NT04 using 16S rRNA gene sequencing, was identified as Enterococcus faecium. In this study, the whole genome of Enterococcus faecium NT04 was sequenced and annotated by bioinformatics analysis tools. Results: Numerous genes encoding the production of diverse metabolic and probiotic properties, such as bacteriocin-like inhibitory substances (Enterocin A and B), cofactors, antioxidants, and vitamins, were confirmed by genomic analysis. There were no pathogenicity islands or plasmid insertions found. This strain is virulent for host colonization rather than invasion. Conclusion: Genomic characteristics of strain NT04 support its potentiality as an anti-oral pathogen probiotic candidate.
