Association of OCT-Derived Drusen Measurements with  AMD-Associated Genotypic SNPs in the Amish Population

Venkata Ramana Murthy Chavali; Bruno Diniz; Jiayan Huang; Gui-Shuang Ying; SriniVas R. Sadda; Dwight Stambolian

doi:10.3390/jcm4020304

Journal of Clinical Medicine (Feb 2015)

Association of OCT-Derived Drusen Measurements with AMD-Associated Genotypic SNPs in the Amish Population

Venkata Ramana Murthy Chavali,
Bruno Diniz,
Jiayan Huang,
Gui-Shuang Ying,
SriniVas R. Sadda,
Dwight Stambolian

Affiliations

Venkata Ramana Murthy Chavali: Department of Ophthalmology, University of Pennsylvania, 313B Stellar-Chance Labs, 422 Curie Blvd., Philadelphia, PA 19104, USA
Bruno Diniz: Doheny Eye Institute, Los Angeles, CA 90033, USA
Jiayan Huang: Center for Preventive Ophthalmology and Biostatistics, Department of Ophthalmology, University of Pennsylvania, Philadelphia, PA 19104, USA
Gui-Shuang Ying: Center for Preventive Ophthalmology and Biostatistics, Department of Ophthalmology, University of Pennsylvania, Philadelphia, PA 19104, USA
SriniVas R. Sadda: Doheny Eye Institute, Los Angeles, CA 90033, USA
Dwight Stambolian: Department of Ophthalmology, University of Pennsylvania, 313B Stellar-Chance Labs, 422 Curie Blvd., Philadelphia, PA 19104, USA

DOI: https://doi.org/10.3390/jcm4020304
Journal volume & issue: Vol. 4, no. 2
pp. 304 – 317

Abstract

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Purpose: To investigate the association of optical coherence tomography (OCT)-derived drusen measures in Amish age-related macular degeneration (AMD) patients with known loci for macular degeneration. Methods: Members of the Old Order Amish community in Pennsylvania ages 50 and older were assessed for drusen area, volume and regions of retinal pigment epithelium (RPE) atrophy using a Cirrus High-Definition OCT. Measurements were obtained in the macula region within a central circle (CC) of 3 mm in diameter and a surrounding perifoveal ring (PR) of 3 to 5 mm in diameter using the Cirrus OCT RPE analysis software. Other demographic information, including age, gender and smoking status, were collected. Study subjects were further genotyped to determine their risk for the AMD-associated SNPs in the SYN3, LIPC, ARMS2, C3, CFB, CETP, CFI and CFH genes using TaqMan genotyping assays. The association of genotypes with OCT measures were assessed using linear trend p-values calculated from univariate and multivariate generalized linear models. Results: 432 eyes were included in the analysis. Multivariate analysis (adjusted by age, gender and smoking status) confirmed the known significant association between AMD and macular drusen with the number of CFH risk alleles for the drusen area (the area increased 0.12 mm2 for a risk allele increase, p < 0.01), drusen volume (the volume increased 0.01 mm3 for a risk allele increase, p ≤ 0.05) and the area of RPE atrophy (the area increased 0.43 mm2 for a risk allele increase, p = 0.003). SYN3 risk allele G is significantly associated with larger area PR (the area increased 0.09 mm2 for a risk allele increase, p = 0.03) and larger drusen volume in the central circle (the volume increased 0.01 mm3 for a risk allele increase, p = 0.04). Conclusion: Among the genotyped SNPs tested, the CFH risk genotype appears to play a major role in determining the drusen phenotype in the Amish AMD population.

Published in Journal of Clinical Medicine

ISSN: 2077-0383 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine
Website: http://www.mdpi.com/journal/jcm

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