Inositol hexakisphosphate induces apoptosis, cell cycle arrest in non-Hodgkin’s Burkitt lymphoma cells and mediates anti-angiogenic, antitumor effects in T-cell lymphoma bearing Swiss albino mice

Abstract

Cancer ranks top as one among the leading cause of non-infectious deaths in humans. Lymphoma occurs as an outcome of uncontrollable proliferation of immune cells. Dalton’s ascites lymphoma (DAL) is a well-established type of non-Hodgkin's T-cell lymphoma of murine models. Inositol hexakisphosphate (IP6) is a phosphate-storage antioxidant present in high volumes among cereals and legumes consumed as a human diet. Based on this background, in the present study, the cytotoxic effects of IP6 were studied in Raji cells, whereas, the antitumor and anti-angiogenic effects were tested in DAL-induced mice. IP6 possessed cytotoxic effects in vitro, induced apoptosis and cell cycle arrest at the G2/M phase in Raji cells. In vivo, eighteen swiss albino male mice were divided into three groups of six mice each. IP6 was effective in prevention of blood vessel formation. The tumor burden reduced considerably as evidenced by body weight and tumor volume. The hematological and biochemical parameters were revived to near-normal in the treatment groups. The antioxidant profile improved significantly after treatment. Reduction in lipid peroxidation and the levels of cellular metabolites indicate the potential of IP6 in the inhibition of DAL-induced intracellular oxidative stress. Altogether, IP6 possessed cytotoxic effects in vitro with anti-angiogenic and antitumor effects in vivo.

Keywords

• Lymphoma
• IP6
• Cytotoxic
• Anti-angiogenic
• Antitumor