Frontiers in Medicine (Sep 2019)

Next-Generation Sequencing for the Diagnosis of Challenging Culture-Negative Endocarditis

  • Manon Kolb,
  • Vladimir Lazarevic,
  • Stéphane Emonet,
  • Stéphane Emonet,
  • Alexandra Calmy,
  • Myriam Girard,
  • Nadia Gaïa,
  • Yannick Charretier,
  • Abdessalam Cherkaoui,
  • Peter Keller,
  • Christoph Huber,
  • Jacques Schrenzel,
  • Jacques Schrenzel,
  • Jacques Schrenzel

DOI
https://doi.org/10.3389/fmed.2019.00203
Journal volume & issue
Vol. 6

Abstract

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Diagnosis of culture-negative infective endocarditis usually implies indirect pathogen identification by serologic or molecular techniques. Clinical metagenomics, relying on next-generation sequencing (NGS) is an emerging approach that allows pathogen identification in challenging situations, as evidenced by a clinical case. We sequenced the DNA extracted from the surgically-removed frozen valve tissue from a patient with suspected infective endocarditis with negative blood and valve cultures. Mapping of the sequence reads against reference genomic sequences, a 16S rRNA gene database and clade-specific marker genes suggested an infection caused by Cardiobacterium hominis.

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