Magnesium Hydroxide Nanoparticles Inhibit the Biofilm Formation of Cariogenic Microorganisms
Kentaro Okamoto,
Daisuke Kudo,
Dao Nguyen Duy Phuong,
Yoshihito Iwamoto,
Koji Watanabe,
Yoshie Yoshioka,
Wataru Ariyoshi,
Ryota Yamasaki
Affiliations
Kentaro Okamoto
Division of Infections and Molecular Biology, Department of Health Promotion, Kyushu Dental University, Kitakyushu, Fukuoka 803-8580, Japan
Daisuke Kudo
SETOLAS Holdings Inc., Hayashida-cho, Sakaide, Kagawa 762-0012, Japan
Dao Nguyen Duy Phuong
SETOLAS Holdings Inc., Hayashida-cho, Sakaide, Kagawa 762-0012, Japan
Yoshihito Iwamoto
SETOLAS Holdings Inc., Hayashida-cho, Sakaide, Kagawa 762-0012, Japan
Koji Watanabe
Division of Developmental Stomatognathic Function Science, Department of Health Promotion, Kyushu Dental University, Kitakyushu, Fukuoka 803-8580, Japan
Yoshie Yoshioka
Division of Infections and Molecular Biology, Department of Health Promotion, Kyushu Dental University, Kitakyushu, Fukuoka 803-8580, Japan
Wataru Ariyoshi
Division of Infections and Molecular Biology, Department of Health Promotion, Kyushu Dental University, Kitakyushu, Fukuoka 803-8580, Japan
Ryota Yamasaki
Division of Infections and Molecular Biology, Department of Health Promotion, Kyushu Dental University, Kitakyushu, Fukuoka 803-8580, Japan
Although various caries-preventive agents have been developed, dental caries is still a leading global disease, mostly caused by biological factors such as mutans streptococci. Magnesium hydroxide nanoparticles have been reported to exhibit antibacterial effects; however, they are rarely used in oral care practical applications. In this study, we examined the inhibitory effect of magnesium hydroxide nanoparticles on biofilm formation by Streptococcus mutans and Streptococcus sobrinus—two typical caries-causing bacteria. Three different sizes of magnesium hydroxide nanoparticles (NM80, NM300, and NM700) were studied, all of which inhibited biofilm formation. The results showed that the nanoparticles were important for the inhibitory effect, which was not influenced by pH or the presence of magnesium ions. We also determined that the inhibition process was mainly contact inhibition and that medium (NM300) and large (NM700) sizes were particularly effective in this regard. The findings of our study demonstrate the potential applications of magnesium hydroxide nanoparticles as caries-preventive agents.
