The Use of Natural Fiber-Rich Food Product Is Safe and Reduces Aberrant Crypt Foci in a Pre-Clinical Model
Luane Aparecida do Amaral,
Taina da Silva Fleming de Almeida,
Gabriel Henrique Oliveira de Souza,
Adrivanio Baranoski,
Rafael Souza Maris,
Felipe Francisco Bittencourt Junior,
Bruna Paola Murino Rafacho,
Antonio Carlos Duenhas Monreal,
Cândida Aparecida Leite Kassuya,
Andréia Conceição Milan Brochado Antoniolli-Silva,
Elisvânia Freitas dos Santos,
Rodrigo Juliano Oliveira
Affiliations
Luane Aparecida do Amaral
Center for Studies in Stem Cells, Cell Therapy and Toxicological Genetics–CeTroGen, University Hospital Maria Aparecida Pedrossian, Federal University of Mato Grosso do Sul, Campo Grande 79080-190, Brazil
Taina da Silva Fleming de Almeida
Postgraduate Program in Biotechnology, Faculty of Pharmaceutical Sciences, Food and Nutrition, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, Brazil
Gabriel Henrique Oliveira de Souza
Faculty of Pharmaceutical Sciences, Food and Nutrition, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, Brazil
Adrivanio Baranoski
Center for Studies in Stem Cells, Cell Therapy and Toxicological Genetics–CeTroGen, University Hospital Maria Aparecida Pedrossian, Federal University of Mato Grosso do Sul, Campo Grande 79080-190, Brazil
Rafael Souza Maris
Clinical Analysis Laboratory, University Center of Grande Dourados, Dourados 79824-900, Brazil
Felipe Francisco Bittencourt Junior
Clinical Analysis Laboratory, University Center of Grande Dourados, Dourados 79824-900, Brazil
Bruna Paola Murino Rafacho
Postgraduate Program in Biotechnology, Faculty of Pharmaceutical Sciences, Food and Nutrition, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, Brazil
Antonio Carlos Duenhas Monreal
Três Lagoas Pedagogical Center, Federal University of Mato Grosso do Sul, Três Lagoas 79600-080, Brazil
Cândida Aparecida Leite Kassuya
Faculty of Health Sciences, University of Grande Dourados, Dourados 79825-900, Brazil
Andréia Conceição Milan Brochado Antoniolli-Silva
Center for Studies in Stem Cells, Cell Therapy and Toxicological Genetics–CeTroGen, University Hospital Maria Aparecida Pedrossian, Federal University of Mato Grosso do Sul, Campo Grande 79080-190, Brazil
Elisvânia Freitas dos Santos
Postgraduate Program in Health and Development in the Midwest Region, Medical School, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, Brazil
Rodrigo Juliano Oliveira
Center for Studies in Stem Cells, Cell Therapy and Toxicological Genetics–CeTroGen, University Hospital Maria Aparecida Pedrossian, Federal University of Mato Grosso do Sul, Campo Grande 79080-190, Brazil
Background: Colorectal cancer is a highly prevalent disease, requiring effective strategies for prevention and treatment. The present research aimed to formulate a natural fiber-rich food product (NFRFP) and to evaluate its safety, toxicogenetics, and effects on aberrant crypt foci induced by 1,2-dimethyl-hydrazine in a preclinical model. Methods: A total of 78 male Wistar rats were distributed in six experimental groups: negative control, positive control (1,2-Dimethylhydrazine—40 mg/Kg), and four groups fed with 10% NFRFP: NFRFP, pre-treatment protocol, simultaneous treatment, and post-treatment protocol. Results: The NFRFP was shown to be a good source of fibers and did not change biometric, biochemical, hematological, and inflammatory parameters, and did not induce signs of toxicity and genotoxicity/carcinogenicity. NFRFP exhibited a chemopreventive effect, in all protocols, with damage reduction (% DR) of 75% in the comet test. NFRFP reduced the incidence of aberrant crypt outbreaks by 49.36% in the post-treatment protocol. Conclusions: The results suggest the applicability of NFRFP in the human diet due to potential production at an industrial scale and easy technological application in different products, since it could be incorporated in food without altering or causing small changes in final product sensory characteristics.