Antimicrobial and Antibiofilm Photodynamic Action of Photosensitizing Nanoassemblies Based on Sulfobutylether-β-Cyclodextrin
Domenico Franco,
Roberto Zagami,
Laura Maria De Plano,
Nina Burduja,
Salvatore Pietro Paolo Guglielmino,
Luigi Monsù Scolaro,
Antonino Mazzaglia
Affiliations
Domenico Franco
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, V. le F. Stagno d’Alcontres 31, 98166 Messina, Italy
Roberto Zagami
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, V. le F. Stagno d’Alcontres 31, 98166 Messina, Italy
Laura Maria De Plano
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, V. le F. Stagno d’Alcontres 31, 98166 Messina, Italy
Nina Burduja
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, V. le F. Stagno d’Alcontres 31, 98166 Messina, Italy
Salvatore Pietro Paolo Guglielmino
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, V. le F. Stagno d’Alcontres 31, 98166 Messina, Italy
Luigi Monsù Scolaro
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, V. le F. Stagno d’Alcontres 31, 98166 Messina, Italy
Antonino Mazzaglia
National Council of Research, Institute for the Study of Nanostructured Materials (CNR-ISMN), URT of Messina c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, V. le F. Stagno d’Alcontres 31, 98166 Messina, Italy
Developing new broad-spectrum antimicrobial strategies, as alternatives to antibiotics and being able to efficiently inactivate pathogens without inducing resistance, is one of the main objectives in public health. Antimicrobial photodynamic therapy (aPDT), based on the light-induced production of reactive oxygen species from photosensitizers (PS), is attracting growing interest in the context of infection treatment, also including biofilm destruction. Due to the limited photostability of free PS, delivery systems are increasingly needed in order to decrease PS photodegradation, thus improving the therapeutic efficacy, as well as to reduce collateral effects on unaffected tissues. In this study, we propose a photosensitizing nanosystem based on the cationic porphyrin 5,10,15,20-tetrakis (N-methyl- 4-pyridyl)-21H,23H-porphyrin (TMPyP), complexed with the commerical sulfobutylether-beta-cyclodextrin (CAPTISOL®), at a 1:50 molar ratio (CAPTISOL®/TMPyP)50_1. Nanoassemblies based on (CAPTISOL®/TMPyP)50_1 with photodynamic features exhibited photo-antimicrobial activity against Gram-negative and Gram-positive bacteria. Moreover, results from P. aeruginosa reveal that CAPTISOL® alone inhibits pyocyanin (PYO) production, also affecting bacterial biofilm formation. Finally, we obtained a synergistic effect of inhibition and destruction of P. aeruginosa biofilm by using the combination of CAPTISOL® and TMPyP.
