Posttranslational modification of Elongation Factor P from Staphylococcus aureus
Alexander Golubev,
Luc Negroni,
Filipp Krasnovid,
Shamil Validov,
Gulnara Yusupova,
Marat Yusupov,
Konstantin Usachev
Affiliations
Alexander Golubev
Laboratory of Structural Biology Institute of Fundamental Medicine and Biology Kazan Federal University Kazan Russian Federation
Luc Negroni
Institut de Génétique et de Biologie Moléculaire et Cellulaire CNRS UMR7104 INSERM U964 Université de Strasbourg Illkirch France
Filipp Krasnovid
Laboratory of Structural Biology Institute of Fundamental Medicine and Biology Kazan Federal University Kazan Russian Federation
Shamil Validov
Laboratory of Structural Biology Institute of Fundamental Medicine and Biology Kazan Federal University Kazan Russian Federation
Gulnara Yusupova
Département de Biologie et de Génomique Structurales Institut de Génétique et de Biologie Moléculaire et Cellulaire CNRS UMR7104 INSERM U964 Université de Strasbourg Illkirch France
Marat Yusupov
Laboratory of Structural Biology Institute of Fundamental Medicine and Biology Kazan Federal University Kazan Russian Federation
Konstantin Usachev
Laboratory of Structural Biology Institute of Fundamental Medicine and Biology Kazan Federal University Kazan Russian Federation
Antibiotic‐resistant Staphylococcus aureus is becoming a major burden on health care systems in many countries, necessitating the identification of new targets for antibiotic development. Elongation Factor P (EF‐P) is a highly conserved elongation protein factor that plays an important role in protein synthesis and bacteria virulence. EF‐P undergoes unique posttranslational modifications in a stepwise manner to function correctly, but experimental information on EF‐P posttranslational modifications is currently lacking for S. aureus. Here, we expressed EF‐P in S. aureus to analyze its posttranslational modifications by mass spectrometry and report experimental proof of 5‐aminopentanol modification of S. aureus EF‐P.
