A moderately thermophilic origin of a novel family of marine group II euryarchaeota from deep ocean
Haodong Liu,
Wei-Wei Liu,
Jose M. Haro-Moreno,
Bu Xu,
Yanfen Zheng,
Jiwen Liu,
Jiwei Tian,
Xiao-Hua Zhang,
Ning-Yi Zhou,
Liping Qin,
Yuanqing Zhu,
Francisco Rodriguez-Valera,
Chuanlun Zhang
Affiliations
Haodong Liu
Shenzhen Key Laboratory of Marine Archaea Geo-Omics, Department of Ocean Science & Engineering, Southern University of Science and Technology, Shenzhen 518055, China; Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 510000, China; CAS Key Laboratory of Crust-Mantle Materials and Environments, University of Science and Technology of China, Hefei 230026, China; School of Global Health, Chinese Centre for Tropical Diseases Research, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
Wei-Wei Liu
State Key Laboratory of Microbial Metabolism & School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
Jose M. Haro-Moreno
Evolutionary Genomics Group, Departamento de Producción Vegetal y Microbiología, Universidad Miguel Hernández, 03550 Alicante, Spain
Bu Xu
Shenzhen Key Laboratory of Marine Archaea Geo-Omics, Department of Ocean Science & Engineering, Southern University of Science and Technology, Shenzhen 518055, China; Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 510000, China
Yanfen Zheng
College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China; Marine Agriculture Research Center, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China
Jiwen Liu
College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
Jiwei Tian
Key Laboratory of Physical Oceanography, Ocean University of China, Qingdao 266100, China
Xiao-Hua Zhang
College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
Ning-Yi Zhou
State Key Laboratory of Microbial Metabolism & School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
Liping Qin
CAS Key Laboratory of Crust-Mantle Materials and Environments, University of Science and Technology of China, Hefei 230026, China
Yuanqing Zhu
Shenzhen Key Laboratory of Marine Archaea Geo-Omics, Department of Ocean Science & Engineering, Southern University of Science and Technology, Shenzhen 518055, China; Shanghai Sheshan National Geophysical Observatory, Shanghai Earthquake Agency, Shanghai 200062, China
Francisco Rodriguez-Valera
Evolutionary Genomics Group, Departamento de Producción Vegetal y Microbiología, Universidad Miguel Hernández, 03550 Alicante, Spain; Laboratory for Theoretical and Computer Studies of Biological Macromolecules and Genomes, Moscow Institute of Physics and Technology, Dolgoprudny, Russia; Corresponding author
Chuanlun Zhang
Shenzhen Key Laboratory of Marine Archaea Geo-Omics, Department of Ocean Science & Engineering, Southern University of Science and Technology, Shenzhen 518055, China; Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 510000, China; Shanghai Sheshan National Geophysical Observatory, Shanghai Earthquake Agency, Shanghai 200062, China; Corresponding author
Summary: Marine group II (MGII) is the most abundant planktonic heterotrophic archaea in the ocean. The evolutionary history of MGII archaea is elusive. In this study, 13 new MGII metagenome-assembled genomes were recovered from surface to the hadal zone in Challenger Deep of the Mariana Trench; four of them from the deep ocean represent a novel group. The optimal growth temperature (OGT) of the common ancestor of MGII has been estimated to be at about 60°C and OGTs of MGIIc, MGIIb, and MGIIa at 47°C–50ºC, 37°C–44ºC, and 30°C–37ºC, respectively, suggesting the adaptation of these species to different temperatures during evolution. The estimated OGT range of MGIIc was supported by experimental measurements of cloned β-galactosidase that showed optimal enzyme activity around 50°C. These results indicate that MGIIc may have originated from a common ancestor that lived in warm or even hot marine environment, such as hydrothermal vents.
