Study on South African Indigenous Teas—Antioxidant Potential, Nutritional Content, and Hypoxia-Induced Cyclooxygenase Inhibition on U87 MG Cell Line
Motlalepula Gilbert Matsabisa,
Asis Bala,
Satyajit Tripathy,
Michelle Mogomane Digashu,
Fanie Rautenbach,
Barsha Dassarma,
Joseph Omorogiuwa Erhabor,
Fernao Castro Braga,
Pulok Kumar Mukherjee,
Minke Tang,
Youngmin Kang
Affiliations
Motlalepula Gilbert Matsabisa
Department of Pharmacology, Faculty of Health Sciences, School of Clinical Medicines, University of Free State, Bloemfontein 9300, South Africa
Asis Bala
Department of Pharmacology, Faculty of Health Sciences, School of Clinical Medicines, University of Free State, Bloemfontein 9300, South Africa
Satyajit Tripathy
Department of Pharmacology, Faculty of Health Sciences, School of Clinical Medicines, University of Free State, Bloemfontein 9300, South Africa
Michelle Mogomane Digashu
Department of Pharmacology, Faculty of Health Sciences, School of Clinical Medicines, University of Free State, Bloemfontein 9300, South Africa
Fanie Rautenbach
Faculty of Health and Wellness Sciences, Oxidative Research Institute, Cape Peninsula University of Technology, Cape Town 7530, South Africa
Barsha Dassarma
Department of Pharmacology, Faculty of Health Sciences, School of Clinical Medicines, University of Free State, Bloemfontein 9300, South Africa
Joseph Omorogiuwa Erhabor
Department of Pharmacology, Faculty of Health Sciences, School of Clinical Medicines, University of Free State, Bloemfontein 9300, South Africa
Fernao Castro Braga
Faculty of Pharmacy, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil
Pulok Kumar Mukherjee
School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, India
Minke Tang
Department of Pharmacology, School of Pharmacy, Beijing University of Chinese Medicines, Beijing 100191, China
Youngmin Kang
Propagation and Production of Traditional Herbal Medicines, Herbal Medicines Resources Centre, Korean Institute of Oriental Medicine, University of Science and Technology, Daejeon 34054, Korea
Background: This study comparatively assessed seven indigenous traditional tea plants on several attributes that included antioxidant, nutritional, caffeine contents, and cyclooxygenase activity. Methodology: Nutritional content of all tea plants were determined for energy, fat, carbohydrates, total sugars, dietary fiber and amino acids. Antioxidant potential and the antioxidant potentiating secondary metabolites were also measured and compared. Further, we investigated the tea plants for any role they would have on cyclooxygenase (COX) activity on cobalt chloride (CoCl2) induced human glioma cell lines (U87MG). Results: The tea plants were found non-cytotoxic at concentrations tested against the human Chang liver and HeK 293 kidney cells and were found to be naturally caffeine free. The lowest and highest extraction yield among the tea plants was 7.1% for B. saligna and 15.48% for L. scaberrimma respectively. On average, the flavonol content was 12 to 8 QE/g, ORAC 800 µmol TE/g, TEAC 150 µmol TE/g, FRAP 155 µmol AAE/g, polyphenols 40 mg GAE/g, flavanols 0.35 mg CE/g, flavonols 12 mg QE/g and total flavonoid content (TFC) 180 µg QE/mg. The COX activity has been found to be inhibited by a dose-dependent manner by L. scaberrimma, B. saligna and L. javanica. Conclusion: The results further support competitive value of tea plants and need for improved and further development.
