Establishment of Carotid Artery Dissection and MRI Findings in a Swine Model

Jing Peng; Min Wu; Desislava Met Doycheva; Yi He; Qiongzhen Huang; Wei Chen; Nathanael Matei; Jun Ding; Kangning Chen; Ningbo Xu; Zhenhua Zhou

doi:10.3389/fneur.2021.669276

Frontiers in Neurology (Jun 2021)

Establishment of Carotid Artery Dissection and MRI Findings in a Swine Model

Jing Peng,
Min Wu,
Desislava Met Doycheva,
Yi He,
Qiongzhen Huang,
Wei Chen,
Nathanael Matei,
Jun Ding,
Kangning Chen,
Ningbo Xu,
Zhenhua Zhou

Affiliations

Jing Peng: Department of Neurology, Southwest Hospital, Third Military Medical University, Chongqing, China
Min Wu: Department of Neurology, Southwest Hospital, Third Military Medical University, Chongqing, China
Desislava Met Doycheva: Departments of Physiology and Pharmacology, Basic Sciences, School of Medicine, Loma Linda University, Loma Linda, CA, United States
Yi He: Department of Microsurgery, Chongqing Hengsheng Surgical Hospital, Chongqing, China
Qiongzhen Huang: Department of Interventional Therapy, Zhujiang Hospital, Southern Medical University, Guangzhou, China
Wei Chen: Department of Radiology, Southwest Hospital, Third Military Medical University, Chongqing, China
Nathanael Matei: Departments of Physiology and Pharmacology, Basic Sciences, School of Medicine, Loma Linda University, Loma Linda, CA, United States
Jun Ding: Department of Ultrasound, Southwest Hospital, Third Military Medical University, Chongqing, China
Kangning Chen: Department of Neurology, Southwest Hospital, Third Military Medical University, Chongqing, China
Ningbo Xu: Department of Interventional Therapy, Zhujiang Hospital, Southern Medical University, Guangzhou, China
Zhenhua Zhou: Department of Neurology, Southwest Hospital, Third Military Medical University, Chongqing, China

DOI: https://doi.org/10.3389/fneur.2021.669276
Journal volume & issue: Vol. 12

Abstract

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Carotid artery dissection (CAD) is the leading cause of ischemic stroke in young patients; however, the etiology and pathophysiology of CAD remain largely unknown. In our study, two types of dissections (length × width: 1.5 cm × 1/3 circumference of intima, Group I, n = 6; or 1.5 cm × 2/3 circumference of intima, Group II, n = 6) were created between the media and intima. Ultrasound (within 2 h after dissection) showed a dissociated intima in the lumen and obstructed blood flow in the surgical area. Digital subtraction angiography (DSA, 72 h after dissection), magnetic resonance imaging (MRI, 72 h after dissection), and hematoxylin–eosin (H&E, 7 days after dissection) staining confirmed stenosis (33.67 ± 5.66%) in Group I and total occlusion in Group II. In 10 out of 12 swine, the CAD model was established using a detacher and balloon dilation, and morphological outcomes (stenosis or occlusion) after CAD were determined by the size of intimal incision.

Published in Frontiers in Neurology

ISSN: 1664-2295 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Internal medicine: Neurosciences. Biological psychiatry. Neuropsychiatry: Neurology. Diseases of the nervous system
Website: https://www.frontiersin.org/journals/neurology/

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