Journal of the Serbian Chemical Society (Jan 2000)

Spectrophotometric determination of fluocortolone in tablets using 1,4-dihydrazinophthalazine

  • Čudina Olivera,
  • Vladimirov Sote,
  • Živanov-Stakić Dobrila,
  • Agbaba Danica

Journal volume & issue
Vol. 65, no. 9
pp. 645 – 648


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The proposed method for the determination of fluocortolone is based on the formation of a stable, yellow coloured hydrazone product with 1,4-dihydrazinophthalazine as the reagent. Heating at 85°C for 2 h was found to be necessary to ensure optimal hydrazone formation in acidified 1-propanol as the solvent. The detection limit was 1.2 µg/ml. This method when applied for the determination of fluocortolone in pharmaceutical formulations gave precise and reproducible results.