Towards organoid culture without Matrigel

Mark T. Kozlowski; Christiana J. Crook; Hsun Teresa Ku

doi:10.1038/s42003-021-02910-8

Communications Biology (Dec 2021)

Towards organoid culture without Matrigel

Mark T. Kozlowski,
Christiana J. Crook,
Hsun Teresa Ku

Affiliations

Mark T. Kozlowski: DEVCOM US Army Research Laboratory, Weapons and Materials Research Directorate, Science of Extreme Materials Division, Polymers Branch
Christiana J. Crook: Department of Translational Research and Cellular Therapeutics, Diabetes and Metabolism Research Institute, City of Hope National Medical Center
Hsun Teresa Ku: Department of Translational Research and Cellular Therapeutics, Diabetes and Metabolism Research Institute, City of Hope National Medical Center

DOI: https://doi.org/10.1038/s42003-021-02910-8
Journal volume & issue: Vol. 4, no. 1
pp. 1 – 15

Abstract

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Kozlowski, Crook, and Ku review current state-of-the-art techniques in culturing and manipulating organoids without the use of EHS-tumor-derived matrix, the most popular of which is Matrigel by Corning. They cover organoid-based applications of different matrix types, ranging from natural sources (decellularized ECM, purified ECM proteins, polysaccharides), synthetic sources (e.g. PEG-based), and recombinant peptide-based systems.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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