mBio
(Feb 2021)
High-Resolution Differentiation of Enteric Bacteria in Premature Infant Fecal Microbiomes Using a Novel rRNA Amplicon
J. Graf,
N. Ledala,
M. J. Caimano,
E. Jackson,
D. Gratalo,
D. Fasulo,
M. D. Driscoll,
S. Coleman,
A. P. Matson
Affiliations
J. Graf
ORCiD University of Connecticut, Department of Molecular and Cell Biology, Storrs, Connecticut, USA
N. Ledala
UConn Health, Department of Pediatrics, Farmington, Connecticut, USA
M. J. Caimano
UConn Health, Department of Pediatrics, Farmington, Connecticut, USA
E. Jackson
Shoreline Biome, Farmington, Connecticut, USA
D. Gratalo
Shoreline Biome, Farmington, Connecticut, USA
D. Fasulo
Pattern Genomics, Madison, Connecticut, USA
M. D. Driscoll
Shoreline Biome, Farmington, Connecticut, USA
S. Coleman
UConn Health, Department of Pediatrics, Farmington, Connecticut, USA
A. P. Matson
UConn Health, Department of Pediatrics, Farmington, Connecticut, USA
DOI
https://doi.org/10.1128/mBio.03656-20
Journal volume & issue
Vol. 12,
no. 1
Abstract
Read online
Achieving strain-level resolution is a major obstacle for source tracking and temporal studies of microbiomes. In this study, we describe a novel deep-sequencing approach that provides species- and strain-level resolution of the neonatal microbiome.
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