YULINK regulates vascular formation in zebrafish and HUVECs

Abstract

Key points 1. Knockdown of YULINK with morpholino in embryos of double transgenic zebrafish exhibited abnormal venous formation. 2. Tube formation and phosphorylated EPHB4 were decreased in YULINK knockdown HUVECs. 3. FLIM-FRET, immunoprecipitation, as well as other imaging technologies showed that YULINK colocalized with endosome related proteins (EPS15, RAB33B and TICAM2) and endosome markers (Clathrin and RHOB). 4. Knockdown of YULINK decreased the internalization of VEGF and VEGFR2 in HUVECs.

Keywords

• YULINK
• Vasculogenesis
• Endocytosis
• Clathrin
• VEGFR2
• VEGF