GC/MS Analysis, Antioxidant Activity, and Antimicrobial Effect of <i>Pelargonium peltatum</i> (Geraniaceae)
Alan-Misael Alonso,
Oscar Kevin Reyes-Maldonado,
Ana María Puebla-Pérez,
Martha Patricia Gallegos Arreola,
Sandra Fabiola Velasco-Ramírez,
Victor Zúñiga-Mayo,
Rosa E. Sánchez-Fernández,
Jorge-Iván Delgado-Saucedo,
Gilberto Velázquez-Juárez
Affiliations
Alan-Misael Alonso
Doctorado en Ciencias en Procesos Biotecnológicos, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Blvd. Marcelino García Barragán #1421, Guadalajara CP 44340, Jalisco, Mexico
Oscar Kevin Reyes-Maldonado
Centro Universitario de Ciencias Exactas e Ingenierías, Laboratorio de Bioquímica Avanzada, Departamento de Química, Universidad de Guadalajara, Blvd. Marcelino García Barragán #1421, Guadalajara CP 44430, Jalisco, Mexico
Ana María Puebla-Pérez
Centro Universitario de Ciencias Exactas e Ingenierías, Departamento de Farmacobiología, Universidad de Guadalajara, Blvd. Marcelino García Barragán #1421, Guadalajara CP 44430, Jalisco, Mexico
Martha Patricia Gallegos Arreola
Centro de Investigación Biomédica de Occidente, División de Genética, I.M.S.S., Sierra Mojada 800, Independencia Oriente, Guadalajara CP 44340, Jalisco, Mexico
Sandra Fabiola Velasco-Ramírez
Centro Universitario de Ciencias Exactas e Ingenierías, Laboratorio de Bioquímica Avanzada, Departamento de Química, Universidad de Guadalajara, Blvd. Marcelino García Barragán #1421, Guadalajara CP 44430, Jalisco, Mexico
Victor Zúñiga-Mayo
Campus Montecillo, CONACyT-Instituto de Fitosanidad, Colegio de Postgraduados, Texcoco CP 56230, Estado de Mexico, Mexico
Rosa E. Sánchez-Fernández
Laboratorio Nacional de Investigación y Servicio Agroalimentario y Forestal (LANISAF), Universidad Autónoma Chapingo, Mexico-Texcoco km 38.5, Texcoco CP 56230, Mexico
Jorge-Iván Delgado-Saucedo
Centro Universitario de Ciencias Exactas e Ingenierías, Departamento de Farmacobiología, Universidad de Guadalajara, Blvd. Marcelino García Barragán #1421, Guadalajara CP 44430, Jalisco, Mexico
Gilberto Velázquez-Juárez
Centro Universitario de Ciencias Exactas e Ingenierías, Laboratorio de Bioquímica Avanzada, Departamento de Química, Universidad de Guadalajara, Blvd. Marcelino García Barragán #1421, Guadalajara CP 44430, Jalisco, Mexico
In recent years, the increase in antibiotic resistance demands searching for new compounds with antimicrobial activity. Phytochemicals found in plants offer an alternative to this problem. The genus Pelargonium contains several species; some have commercial use in traditional medicine such as P. sinoides, and others such as P. peltatum are little studied but have promising potential for various applications such as phytopharmaceuticals. In this work, we characterized the freeze-dried extracts (FDEs) of five tissues (root, stem, leaf, and two types of flowers) and the ethyl acetate fractions from leaf (Lf-EtOAc) and flower (Fwr-EtOAc) of P. peltatum through the analysis by thin-layer chromatography (T.L.C.), gas chromatography coupled to mass spectrometry (GC-MS), phytochemicals quantification, antioxidant capacity, and antimicrobial activity. After the first round of analysis, it was observed that the FDE-Leaf and FDE-Flower showed higher antioxidant and antimicrobial activities compared to the other FDEs, for which FDE-Leaf and FDE-Flower were fractionated and analyzed in a second round. The antioxidant activity determined by ABTS showed that Lf-EtOAc and Fwr-EtOAc had the lowest IC50 values with 27.15 ± 1.04 and 28.11 ± 1.3 µg/mL, respectively. The content of total polyphenols was 264.57 ± 7.73 for Lf-EtOAc and 105.39 ± 4.04 mg G.A./g FDE for Fwr-EtOAc. Regarding the content of flavonoid, Lf-EtOAc and Fw-EtOAc had the highest concentration with 34.4 ± 1.06 and 29.45 ± 1.09 mg Q.E./g FDE. In addition, the minimum inhibitory concentration (M.I.C.) of antimicrobial activity was evaluated: Lf-EtOAc and Fwr-EtOAc were effective at 31.2 µg/mL for Staphylococcus aureus and 62.5 µg/mL for Salmonella enterica, while for the Enterococcus feacalis strain, Fwr-EtOAc presented 31.2 µg/mL of M.I.C. According to the GC-MS analysis, the main compounds were 1,2,3-Benzenetriol (Pyrogallol), with 77.38% of relative abundance in the Lf-EtOAc and 71.24% in the Fwr-EtOAc, followed by ethyl gallate (13.10%) in the Fwr-EtOAc and (Z)-9-Octadecenamide (13.63% and 6.75%) in both Lf-EtOAc and Fwr-EtOAc, respectively.
