Estimation of titers of antibody against Pasteurella multocida in cattle vaccinated with haemorrhagic septicemia alum precipitated vaccine

Abstract

Aim: The present study was carried out in 100 cattle to assess the antibody response to Haemorrhagic Septicaemia alum precipitated vaccine by Microtiter Agglutination Test (MAT), Indirect Haemaaglutination Assay (IHA) and Monoclonal Antibody based Indirect Enzyme Linked Immunosorbent Assay (ELISA). Materials and Methods: One hundred cattle from a local gaushala of Ludhiana were vaccinated with alum precipitated Haemorrhagic Septicaemia vaccine. Serum was collected at 0, 42, 84 and 128 days post immunization and antibody titers at different stages were estimated by MAT, IHA and ELISA. Results: The animals exhibited the classical pattern of humoral immune response with gradual increase and achievement of peak antibody titers plateau by 42DPI and gradual decline by 128 DPI. The IHA titers in cattle were significantly higher (P<0.05) at 42 days post immunization than the corresponding MAT titers on the same day. ELISA titers were significantly higher (P<0.05) than MAT and IHA titers at 42 DPI. IHA was found to be more sensitive than MAT, and the titers were higher by ELISA than by MAT and IHA throughout the observation period. Conclusion: The results indicate that animals vaccinated with commercial alum precipitated HS vaccine could not develop and sustain adequate levels of antibody for long duration.

Keywords

• antibody titers
• haemorrhagic septicaemia
• Indirect Haemaaglutination Assay