pH-driven continuous stem cell production with enhanced regenerative capacity from polyamide/chitosan surfaces

Chia-Hsiang Yen; Nai-Chen Cheng; Hao-Ying Hsieh; Ching-Wen Tsai; An-Li Lee; Chien-Yi Lu; Yin-Tzu Chen; Tai-Horng Young

Materials Today Bio (Feb 2023)

pH-driven continuous stem cell production with enhanced regenerative capacity from polyamide/chitosan surfaces

Chia-Hsiang Yen,
Nai-Chen Cheng,
Hao-Ying Hsieh,
Ching-Wen Tsai,
An-Li Lee,
Chien-Yi Lu,
Yin-Tzu Chen,
Tai-Horng Young

Affiliations

Chia-Hsiang Yen: Department of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, No. 1, Sec. 1, Jen-Ai Rd., Taipei, 100, Taiwan
Nai-Chen Cheng: Department of Surgery, National Taiwan University Hospital and College of Medicine, No. 7, Chung-Shan S Rd., Taipei, 100, Taiwan
Hao-Ying Hsieh: Department of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, No. 1, Sec. 1, Jen-Ai Rd., Taipei, 100, Taiwan; Department of Dentistry, National Taiwan University Hospital, No. 7, Chung-Shan S Rd., Taipei, 100, Taiwan
Ching-Wen Tsai: Taiwan Instrument Research Institute, National Applied Research Laboratories, No. 20, R&D Rd. VI, Hsinchu Science Park, Hsinchu, 300, Taiwan
An-Li Lee: Department of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, No. 1, Sec. 1, Jen-Ai Rd., Taipei, 100, Taiwan; Department of Plastic and Reconstructive Surgery, MacKay Memorial Hospital, No. 92, Sec. 2, Chung-Shan N Rd., Taipei, 104, Taiwan; Department of Medicine, MacKay Medical College, No. 46, Sec. 3, Zhong-Zheng Rd., New Taipei City, 252, Taiwan
Chien-Yi Lu: Department of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, No. 1, Sec. 1, Jen-Ai Rd., Taipei, 100, Taiwan
Yin-Tzu Chen: Department of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, No. 1, Sec. 1, Jen-Ai Rd., Taipei, 100, Taiwan
Tai-Horng Young: Department of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, No. 1, Sec. 1, Jen-Ai Rd., Taipei, 100, Taiwan; Department of Biomedical Engineering, National Taiwan University Hospital, No. 7, Chung-Shan S Rd., Taipei, 100, Taiwan; Corresponding author. Department of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, No. 1, Sec. 1, Jen-Ai Rd., Taipei, 100, Taiwan.

Journal volume & issue: Vol. 18
p. 100514

Abstract

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Adipose-derived stem cells (ASCs) have raised significant interest for their potential therapeutic applications in regenerative medicine. However, ASCs usually suffer from decreased pluripotency and functional plasticity during in vitro expansion. Herein, this study sought to develop a continuous cell production system that can mass-produce ASCs with sustained regenerative capacity. The strategy was blending pH-responsive chitosan (CS) with polyamide-66 (PA) to generate combined surface properties with controllable cell growth/detachment ability to achieve a repeated cell production process. From the collected data, all the polymer blends were capable of completing a minimum of four consecutive production cycles, wherein the PA17CS blend (PA:CS = 1:7) outperformed with respect to the working effectiveness (average cell detachment ratio= 88%) and the cell viability. Compared to the trypsin-based method, ASCs harvested from PA17CS exhibited superior stemness characteristics along with SDF-1-mediated CXCR4 chemotactic response for stem cell homing. Moreover, injection of ASCs generated from PA17CS blend could more effectively induce neovascularization and protect skin ﬂaps during an ischemic injury in a rat model.

Published in Materials Today Bio

ISSN: 2590-0064 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Medicine: Medicine (General); Science: Biology (General)
Website: https://www.journals.elsevier.com/materials-today-bio

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