Fayixue Zazhi (Oct 2023)

Cross-Platform Application of DNA Methylation Age Estimation Model in Eastern Chinese Han Population

  • Zi-wei WANG, Cheng-tao LI, Xi-ling LIU

DOI
https://doi.org/10.12116/j.issn.1004-5619.2022.520405
Journal volume & issue
Vol. 39, no. 5
pp. 441 – 446

Abstract

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Objective To evaluate the forensic application value of an age estimation model based on DNA methylation in eastern Chinese Han population, and to provide a theoretical basis for exploring age estimation models suitable for different detection platforms. Methods According to the 6 age-related methylation sites in the published blood DNA methylation age estimation models of Chinese Han population, the DNA methylation level of 48 samples was detected by pyrosequencing and next-generation sequencing (NGS). After submitting DNA methylation levels to the age estimation model, the DNA methylation ages were predicted and compared with their real ages. Results The 6 DNA methylation sites in both detection techniques were age-related, with an R2 of 0.85 and a median absolute deviation (MAD) of 4.81 years when using pyrosequencing; with an R2 of 0.84 and MAD of 4.41 years when using NGS. Conclusion The blood DNA methylation age estimation model can be used under pyrosequencing and multi-purpose regional methylation enrichment sequencing technology based on NGS and it can accurately estimate the age.

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