High-throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbers

Abstract

Abstract Background Chromatin immunoprecipitation coupled to sequencing (ChIP-seq) is widely used to map histone modifications and transcription factor binding on a genome-wide level. Results We present high-throughput ChIPmentation (HT-ChIPmentation) that eliminates the need for DNA purification prior to library amplification and reduces reverse-crosslinking time from hours to minutes. Conclusions The resulting workflow is easily established, extremely rapid, and compatible with requirements for very low numbers of FACS sorted cells, high-throughput applications and single day data generation.

Keywords

• Chromatin immunoprecipitation
• ChIP-seq
• ChIPmentation
• High-throughput genomics
• Epigenetics