Molecular Detection of Ureaplasma urealyticum and Chlamydia trachomatis in Women with Endometriosis

Abstract

Background and Aims: Endometriosis is considered to be one of the most common women's diseases in the world. The exact cause of endometriosis is unknown. In this study, the bacterial agents were considered as the probable factors influencing this disease and the frequency of two bacteria U. urealyticum and C. trachomatis was studied in this disease. Materials and Methods: This study was performed on Pap smear samples of 50 women with endometriosis, 48 ​​healthy women referred to hospitals in north of Tehran. The recommend samples were transferred to the laboratory, their DNA was extracted, and PCR method was performed. They were analyzed by descriptive statistics. Results: Performing PCR test for U. urealyticum detection among the pap smears of people with endometriosis and non_endometriosis, 27 samples (54%) and 25 samples (52%) were positive respectively but none of the samples had contamination with C. trachomatis. The highest prevalence of U. urealyticum in both endometriosis and healthy subjects was observed in the age group of 30-35 years. Conclusion: The prevalence of U. urealyticum in patients with endometriosis and healthy subjects (non-sedation) was relatively similar and the same as normal flora. There was no infection with C. trachomatis in these individuals, therefore there was no significant association between this bacterium and endometriosis. For more accurate results such studies should be done in a higher statistical society.

Keywords

• pcr
• u. urealyticum
• c. trachomatis
• endometriosis