Rapid diagnostic testing method to detect ROB β-lactamase gene in Neisseria meningitidis

Andrew Peifer; Anna Kidney; Geetha Nattanmai; Kate Wahl; Sherly Jose; Elizabeth Owuor; Linnell Randall; Erin Klingbeil; Kimberlee A. Musser; Kara Mitchell

Molecular and Cellular Probes (Feb 2025)

Rapid diagnostic testing method to detect ROB β-lactamase gene in Neisseria meningitidis

Andrew Peifer,
Anna Kidney,
Geetha Nattanmai,
Kate Wahl,
Sherly Jose,
Elizabeth Owuor,
Linnell Randall,
Erin Klingbeil,
Kimberlee A. Musser,
Kara Mitchell

Affiliations

Andrew Peifer: Corresponding author.; Wadsworth Center, New York State Department of Health, PO Box 22002, Albany, NY, 12201-2002, USA
Anna Kidney: Wadsworth Center, New York State Department of Health, PO Box 22002, Albany, NY, 12201-2002, USA
Geetha Nattanmai: Wadsworth Center, New York State Department of Health, PO Box 22002, Albany, NY, 12201-2002, USA
Kate Wahl: Wadsworth Center, New York State Department of Health, PO Box 22002, Albany, NY, 12201-2002, USA
Sherly Jose: Wadsworth Center, New York State Department of Health, PO Box 22002, Albany, NY, 12201-2002, USA
Elizabeth Owuor: Wadsworth Center, New York State Department of Health, PO Box 22002, Albany, NY, 12201-2002, USA
Linnell Randall: Wadsworth Center, New York State Department of Health, PO Box 22002, Albany, NY, 12201-2002, USA
Erin Klingbeil: Wadsworth Center, New York State Department of Health, PO Box 22002, Albany, NY, 12201-2002, USA
Kimberlee A. Musser: Wadsworth Center, New York State Department of Health, PO Box 22002, Albany, NY, 12201-2002, USA
Kara Mitchell: Wadsworth Center, New York State Department of Health, PO Box 22002, Albany, NY, 12201-2002, USA

Journal volume & issue: Vol. 79
p. 102000

Abstract

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blaROB-1 is the only widely found β-lactamase in Neisseria meningitidis, and its presence is on the rise. To enhance our bacterial meningitis testing procedure, we clinically validated a real-time PCR assay to rapidly detect the blaROB gene and predict drug resistance in Neisseria meningitidis. A screen of 101 clinical isolates and 37 clinical specimens of blood and cerebrospinal fluid received between January 2018 and June 2024 found 8 isolates and 2 cerebrospinal fluid specimens that were positive for blaROB.

Published in Molecular and Cellular Probes

ISSN: 0890-8508 (Print); 1096-1194 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General); Medicine
Website: https://www.sciencedirect.com/journal/molecular-and-cellular-probes

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Abstract

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