TMEM163 Regulates ATP-Gated P2X Receptor and Behavior

Elizabeth J. Salm; Patrick J. Dunn; Lili Shan; Miwako Yamasaki; Nathalie M. Malewicz; Taisuke Miyazaki; Joongkyu Park; Akio Sumioka; R. Richard L. Hamer; Wei-Wu He; Megumi Morimoto-Tomita; Robert H. LaMotte; Susumu Tomita

Cell Reports (Jun 2020)

TMEM163 Regulates ATP-Gated P2X Receptor and Behavior

Elizabeth J. Salm,
Patrick J. Dunn,
Lili Shan,
Miwako Yamasaki,
Nathalie M. Malewicz,
Taisuke Miyazaki,
Joongkyu Park,
Akio Sumioka,
R. Richard L. Hamer,
Wei-Wu He,
Megumi Morimoto-Tomita,
Robert H. LaMotte,
Susumu Tomita

Affiliations

Elizabeth J. Salm: Department of Cellular and Molecular Physiology, Department of Neuroscience, Program in Cellular Neuroscience, Neurodegeneration and Repair, The Yale Kavli Institute, Yale University School of Medicine, New Haven, CT 06520, USA; Interdepartmental Neuroscience Program, Yale University School of Medicine, New Haven, CT 06520, USA
Patrick J. Dunn: Department of Cellular and Molecular Physiology, Department of Neuroscience, Program in Cellular Neuroscience, Neurodegeneration and Repair, The Yale Kavli Institute, Yale University School of Medicine, New Haven, CT 06520, USA
Lili Shan: Department of Cellular and Molecular Physiology, Department of Neuroscience, Program in Cellular Neuroscience, Neurodegeneration and Repair, The Yale Kavli Institute, Yale University School of Medicine, New Haven, CT 06520, USA
Miwako Yamasaki: Department of Cellular and Molecular Physiology, Department of Neuroscience, Program in Cellular Neuroscience, Neurodegeneration and Repair, The Yale Kavli Institute, Yale University School of Medicine, New Haven, CT 06520, USA; Department of Anatomy, Faculty of Medicine, Hokkaido University, Sapporo, Japan
Nathalie M. Malewicz: Department of Anesthesiology, Yale University School of Medicine, New Haven, CT 06520, USA
Taisuke Miyazaki: Department of Cellular and Molecular Physiology, Department of Neuroscience, Program in Cellular Neuroscience, Neurodegeneration and Repair, The Yale Kavli Institute, Yale University School of Medicine, New Haven, CT 06520, USA; Department of Anatomy, Faculty of Medicine, Hokkaido University, Sapporo, Japan
Joongkyu Park: Department of Cellular and Molecular Physiology, Department of Neuroscience, Program in Cellular Neuroscience, Neurodegeneration and Repair, The Yale Kavli Institute, Yale University School of Medicine, New Haven, CT 06520, USA
Akio Sumioka: Department of Cellular and Molecular Physiology, Department of Neuroscience, Program in Cellular Neuroscience, Neurodegeneration and Repair, The Yale Kavli Institute, Yale University School of Medicine, New Haven, CT 06520, USA
R. Richard L. Hamer: OriGene Technologies, Inc., Rockville, MD 20850, USA
Wei-Wu He: OriGene Technologies, Inc., Rockville, MD 20850, USA
Megumi Morimoto-Tomita: Department of Cellular and Molecular Physiology, Department of Neuroscience, Program in Cellular Neuroscience, Neurodegeneration and Repair, The Yale Kavli Institute, Yale University School of Medicine, New Haven, CT 06520, USA
Robert H. LaMotte: Department of Anesthesiology, Yale University School of Medicine, New Haven, CT 06520, USA
Susumu Tomita: Department of Cellular and Molecular Physiology, Department of Neuroscience, Program in Cellular Neuroscience, Neurodegeneration and Repair, The Yale Kavli Institute, Yale University School of Medicine, New Haven, CT 06520, USA; Interdepartmental Neuroscience Program, Yale University School of Medicine, New Haven, CT 06520, USA; Corresponding author

Journal volume & issue: Vol. 31, no. 9

Abstract

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Summary: Fast purinergic signaling is mediated by ATP and ATP-gated ionotropic P2X receptors (P2XRs), and it is implicated in pain-related behaviors. The properties exhibited by P2XRs vary between those expressed in heterologous cells and in vivo. Several modulators of ligand-gated ion channels have recently been identified, suggesting that there are P2XR functional modulators in vivo. Here, we establish a genome-wide open reading frame (ORF) collection and perform functional screening to identify modulators of P2XR activity. We identify TMEM163, which specifically modulates the channel properties and pharmacology of P2XRs. We also find that TMEM163 is required for full function of the neuronal P2XR and a pain-related ATP-evoked behavior. These results establish TMEM163 as a critical modulator of P2XRs in vivo and a potential target for the discovery of drugs for treating pain.

Published in Cell Reports

ISSN: 2211-1247 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: http://www.cell.com/cell-reports/home

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