Nitrocellulose redox permanganometry: A simple method for reductive capacity assessment
Jan Homolak,
Ivan Kodvanj,
Ana Babic Perhoc,
Davor Virag,
Ana Knezovic,
Jelena Osmanovic Barilar,
Peter Riederer,
Melita Salkovic-Petrisic
Affiliations
Jan Homolak
Department of Pharmacology, University of Zagreb School of Medicine, Zagreb, Croatia; Research Centre of Excellence, Institute of Fundamental Clinical and Translational Neuroscience, Croatian Institute for Brain Research, University of Zagreb School of Medicine, Zagreb, Croatia; Corresponding author at: Department of Pharmacology, University of Zagreb School of Medicine, Zagreb, Croatia.
Ivan Kodvanj
Department of Pharmacology, University of Zagreb School of Medicine, Zagreb, Croatia; Research Centre of Excellence, Institute of Fundamental Clinical and Translational Neuroscience, Croatian Institute for Brain Research, University of Zagreb School of Medicine, Zagreb, Croatia
Ana Babic Perhoc
Department of Pharmacology, University of Zagreb School of Medicine, Zagreb, Croatia; Research Centre of Excellence, Institute of Fundamental Clinical and Translational Neuroscience, Croatian Institute for Brain Research, University of Zagreb School of Medicine, Zagreb, Croatia
Davor Virag
Department of Pharmacology, University of Zagreb School of Medicine, Zagreb, Croatia; Research Centre of Excellence, Institute of Fundamental Clinical and Translational Neuroscience, Croatian Institute for Brain Research, University of Zagreb School of Medicine, Zagreb, Croatia
Ana Knezovic
Department of Pharmacology, University of Zagreb School of Medicine, Zagreb, Croatia; Research Centre of Excellence, Institute of Fundamental Clinical and Translational Neuroscience, Croatian Institute for Brain Research, University of Zagreb School of Medicine, Zagreb, Croatia
Jelena Osmanovic Barilar
Department of Pharmacology, University of Zagreb School of Medicine, Zagreb, Croatia; Research Centre of Excellence, Institute of Fundamental Clinical and Translational Neuroscience, Croatian Institute for Brain Research, University of Zagreb School of Medicine, Zagreb, Croatia
Peter Riederer
Center of Mental Health, Department of Psychiatry, Psychosomatics and Psychotherapy, University Hospital Würzburg, Würzburg, Germany; Department and Research Unit of Psychiatry, Institute of Clinical Research, University of Southern Denmark, Odense, Denmark
Melita Salkovic-Petrisic
Department of Pharmacology, University of Zagreb School of Medicine, Zagreb, Croatia; Research Centre of Excellence, Institute of Fundamental Clinical and Translational Neuroscience, Croatian Institute for Brain Research, University of Zagreb School of Medicine, Zagreb, Croatia
We propose a rapid, simple, and robust method for measurement of the reductive capacity of liquid and solid biological samples based on potassium permanganate reduction followed by trapping of manganese dioxide precipitate on a nitrocellulose membrane. Moreover, we discuss how nitrocellulose redox permanganometry (NRP) can be used for high-throughput analysis of biological samples and present HistoNRP, its modification used for detailed analysis of reductive capacity spatial distribution in tissue with preserved anatomical relations. • NRP is a rapid, cost-effective, and simple method for reductive capacity assessment • NRP is compatible with a high-throughput screening of solid and liquid biological samples • HistoNRP exploits passive diffusion slice print blotting for reductive capacity spatial analysis
