Supramolecular Tandem Assay for Pyridoxal‐5′‐phosphate by the Reporter Pair of Guanidinocalix[5]Arene and Fluorescein
Yu‐Xin Yue,
Yong Kong,
Fan Yang,
Zhe Zheng,
Xin‐Yue Hu,
Dong‐Sheng Guo
Affiliations
Yu‐Xin Yue
College of Chemistry, State Key Laboratory of Elemento-Organic Chemistry, Key Laboratory of Functional Polymer Materials (Ministry of Education), Tianjin Key Laboratory of Biosensing and Molecular Recognition Nankai University Tianjin 300071 P. R. China
Yong Kong
Research Institute of Petroleum Engineering, Sinopec Beijing 100101 P. R. China
Fan Yang
Research Institute of Petroleum Engineering, Sinopec Beijing 100101 P. R. China
Zhe Zheng
College of Chemistry, State Key Laboratory of Elemento-Organic Chemistry, Key Laboratory of Functional Polymer Materials (Ministry of Education), Tianjin Key Laboratory of Biosensing and Molecular Recognition Nankai University Tianjin 300071 P. R. China
Xin‐Yue Hu
College of Chemistry, State Key Laboratory of Elemento-Organic Chemistry, Key Laboratory of Functional Polymer Materials (Ministry of Education), Tianjin Key Laboratory of Biosensing and Molecular Recognition Nankai University Tianjin 300071 P. R. China
Dong‐Sheng Guo
College of Chemistry, State Key Laboratory of Elemento-Organic Chemistry, Key Laboratory of Functional Polymer Materials (Ministry of Education), Tianjin Key Laboratory of Biosensing and Molecular Recognition Nankai University Tianjin 300071 P. R. China
Abstract Guanidinocalix[5]arene and fluorescein reporter pair has been chosen to set up a supramolecular tandem assay principle based on the differential recognition of pyridoxal‐5′‐phosphate (the substrate of alkaline phosphatase, ALP), pyridoxal (the product of ALP) and phosphate (the product of ALP). This supramolecular tandem assay system offers an opportunity to monitor the activity of ALP in a label‐free, continuous, and real‐time manner. More importantly, a calibration curve can be given for selective and quantitative detection of pyridoxal‐5′‐phosphate (biomarker for several diseases).
