OncoTargets and Therapy (Feb 2021)
Novel lncRNA LINC00941 Promotes Proliferation and Invasion of Colon Cancer Through Activation of MYC
Abstract
Lin Chang,1 Dongmin Zhou,1 Suxia Luo2 1Intensive Care Unit, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, Henan Province, People’s Republic of China; 2Medical Oncology, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, Henan Province, People’s Republic of ChinaCorrespondence: Suxia LuoMedical Oncology, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, Henan Province, People’s Republic of ChinaEmail [email protected]: We conducted the study to elucidate how LncRNA LINC00941 affects colon cancer progression and its possible regulatory mechanism.Methods: The expression level of LINC00941 in colon cancer tissues and cells was detected by qRT-PCR. The function of LINC00941 on colon cancer cell proliferation, migration, and invasion was detected by CCK-8 and Transwell assay respectively. The target interactions among LINC00941, miR-205-5p, and MYC were further confirmed by dual-luciferase reporter gene assays and RNA pull-down experiments. Meanwhile, in vivo experiments were carried out to study the role of LINC00941 in the xenotransplantation model.Results: LINC00941 expression level was elevated in colon cancer tissues and cells. LINC00941 overexpression accelerated proliferation, migration, and invasion of colon cancer cells, while the LINC00941 knockdown showed the opposite results. In addition, LINC00941 regulated the expression of MYC by sponging miR-205-5p as a competitive endogenous RNA, and miR-205-5p knockdown reversed the tumor inhibition of LINC00941 knockdown on colon cancer cells. Xenograft model assay confirmed that LINC00941 silencing could inhibit colon cancer cell growth and metastasis.Conclusion: LINC00941 may markedly promote colon cancer progression by acting on the miR-205-5p/MYC axis as a ceRNA, which offers novel clues for lncRNA to guide the treatment and prognosis of colon cancer.Keywords: colon cancer, LINC00941, miR-205-5p, MYC, ceRNA
