Journal of Lipid Research (Aug 1978)

A rapid biosynthetic method for the preparation of radioactive phosphatidyl-CMP(CDP-diacylglycerol) of high specific activity.

  • J Eichberg,
  • G Hauser

DOI
https://doi.org/10.1016/s0022-2275(20)41281-7
Journal volume & issue
Vol. 19, no. 6
pp. 778 – 783

Abstract

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A procedrue is described for the preparation of [32P]phosphatidyl-CMP(CDP-diacylglycerol) from rat pineal glands incubated with [32P]orthophosphate and DL-propranolol. The product is 95% radiopure and a high specific acitivity. The yield of liponucleotide is 0.4–0.9 micron Ci/mCi of [32P]orthophosphate in the medium. The same method can also be used for the biosynthesis and purification of [3H]phosphatidyl-CMP when [3H]cytidine is the precursor.