Transcriptome analysis of biotic and abiotic stress mediated by the suction of brown planthopper in two rice cultivars
Changyan Li,
Wenjun Zha,
Jianyu Wang,
Yan Wu,
Shaojie Shi,
Huiying Wang,
Sanhe Li,
Bian Wu,
Kai Liu,
Junxiao Chen,
Huashan Xu,
Peide Li,
Zhijun Chen,
Kai Liu,
Guocai Yang,
Bingliang Wan,
Lei Zhou,
Aiqing You
Affiliations
Changyan Li
Food Crops Institute, Hubei Academy of Agricultural Sciences, Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Wuhan 430064, PR China
Wenjun Zha
Food Crops Institute, Hubei Academy of Agricultural Sciences, Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Wuhan 430064, PR China
Jianyu Wang
Food Crops Institute, Hubei Academy of Agricultural Sciences, Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Wuhan 430064, PR China
Yan Wu
Food Crops Institute, Hubei Academy of Agricultural Sciences, Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Wuhan 430064, PR China
Shaojie Shi
Food Crops Institute, Hubei Academy of Agricultural Sciences, Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Wuhan 430064, PR China
Huiying Wang
Food Crops Institute, Hubei Academy of Agricultural Sciences, Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Wuhan 430064, PR China
Sanhe Li
Food Crops Institute, Hubei Academy of Agricultural Sciences, Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Wuhan 430064, PR China
Bian Wu
Food Crops Institute, Hubei Academy of Agricultural Sciences, Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Wuhan 430064, PR China
Kai Liu
Food Crops Institute, Hubei Academy of Agricultural Sciences, Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Wuhan 430064, PR China
Junxiao Chen
Food Crops Institute, Hubei Academy of Agricultural Sciences, Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Wuhan 430064, PR China
Huashan Xu
Food Crops Institute, Hubei Academy of Agricultural Sciences, Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Wuhan 430064, PR China
Peide Li
Food Crops Institute, Hubei Academy of Agricultural Sciences, Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Wuhan 430064, PR China
Zhijun Chen
Food Crops Institute, Hubei Academy of Agricultural Sciences, Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Wuhan 430064, PR China
Kai Liu
Food Crops Institute, Hubei Academy of Agricultural Sciences, Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Wuhan 430064, PR China
Guocai Yang
Food Crops Institute, Hubei Academy of Agricultural Sciences, Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Wuhan 430064, PR China
Bingliang Wan
Food Crops Institute, Hubei Academy of Agricultural Sciences, Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Wuhan 430064, PR China; Corresponding authors.
Lei Zhou
Food Crops Institute, Hubei Academy of Agricultural Sciences, Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Wuhan 430064, PR China; Hubei Hongshan Laboratory, Wuhan 430070, PR China; Corresponding authors.
Aiqing You
Food Crops Institute, Hubei Academy of Agricultural Sciences, Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Wuhan 430064, PR China; Hubei Hongshan Laboratory, Wuhan 430070, PR China; Corresponding authors.
RH (Rathu Heenati), an indica rice cultivar from Sri Lanka, is highly resistant to several different biotypes of brown planthopper (Nilaparvata lugens Stål, BPH). In contrast, another indica rice cultivar, TN1 (Taichung Native 1), is highly susceptible to all biotypes of BPH. To reveal the molecular biological mechanism of biotic and abiotic stress mediated by BPH suction in two rice cultivars, RH and TN1, the genome-wide gene expressional profiling of these two rice varieties at 6 h after BPH infestation and needle puncturing was conducted by performing transcriptome analysis. In total, six samples (T6C, T6N, T6B, R6C, R6N, and R6B) were collected after performing different treatments for this expressional analysis. The results provide important information for the further isolation of BPH-resistance genes from RH as well as research on the mechanism of BPH resistance in RH. The transcriptome results revealed that TN1 (a BPH-susceptible rice variety) and RH (a BPH-resistant rice variety) harbor different pathways that respond to the physical injury of acupuncture and BPH infestation. The susceptible variety TN1 is more sensitive to the physical damage caused by acupuncture, whereas in the resistant variety RH, the response to insect damage is more rapid and direct. By comparing the gene changes of various plant hormones between the two varieties under different stresses, it was found that, in addition to the traditional SA and ET pathways related to the feeding induction of piercing-sucking pests, the expression patterns of JAZ-related genes in the JA signaling pathway also changed under two stresses (acupuncture induction and brown planthopper feeding induction), while the IAA hormone pathway-related genes in the susceptible variety TN1 also changed significantly after brown planthopper feeding. Furthermore, the results of this study help us to understand the biological process of rice responses to BPH and provide a promising direction to identify BPH-resistance-related genes in RH.
