Salivary Microbiome in Adenoid Cystic Carcinoma Detected by 16S rRNA Sequencing and Shotgun Metagenomics

Qian Jiang; Qian Jiang; Qian Jiang; Xing Liu; Xing Liu; Xing Liu; Qifen Yang; Qifen Yang; Qifen Yang; Liang Chen; Liang Chen; Liang Chen; Deqin Yang; Deqin Yang; Deqin Yang

doi:10.3389/fcimb.2021.774453

Frontiers in Cellular and Infection Microbiology (Dec 2021)

Salivary Microbiome in Adenoid Cystic Carcinoma Detected by 16S rRNA Sequencing and Shotgun Metagenomics

Qian Jiang,
Qian Jiang,
Qian Jiang,
Xing Liu,
Xing Liu,
Xing Liu,
Qifen Yang,
Qifen Yang,
Qifen Yang,
Liang Chen,
Liang Chen,
Liang Chen,
Deqin Yang,
Deqin Yang,
Deqin Yang

Affiliations

Qian Jiang: Stomatological Hospital of Chongqing Medical University, Chongqing, China
Qian Jiang: Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing, China
Qian Jiang: Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing, China
Xing Liu: Stomatological Hospital of Chongqing Medical University, Chongqing, China
Xing Liu: Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing, China
Xing Liu: Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing, China
Qifen Yang: Stomatological Hospital of Chongqing Medical University, Chongqing, China
Qifen Yang: Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing, China
Qifen Yang: Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing, China
Liang Chen: Stomatological Hospital of Chongqing Medical University, Chongqing, China
Liang Chen: Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing, China
Liang Chen: Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing, China
Deqin Yang: Stomatological Hospital of Chongqing Medical University, Chongqing, China
Deqin Yang: Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing, China
Deqin Yang: Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing, China

DOI: https://doi.org/10.3389/fcimb.2021.774453
Journal volume & issue: Vol. 11

Abstract

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Microorganisms are confirmed to be closely related to the occurrence and development of cancers in human beings. However, there has been no published report detailing relationships between the oral microbiota and salivary adenoid cystic carcinoma (SACC). In this study, unstimulated saliva was collected from 13 SACC patients and 10 healthy controls. The microbial diversities, compositions and functions were comprehensively analyzed after 16S rRNA sequencing and whole-genome shotgun metagenomic sequencing. The alpha diversity showed no significant difference between SACC patients and healthy controls, while beta diversity showed a separation trend. The SACC patients showed higher abundances of Streptococcus and Rothia, while Prevotella and Alloprevotella were more abundant in healthy controls. The prevalent KEGG pathways, carbohydrate-active enzymes, antibiotic resistances and virulence factors as well as the biomarkers in SACC were determined by functional gene analysis. Our study preliminarily investigated the salivary microbiome of SACC patients compared with healthy controls and might be the basis for further studies on novel diagnostic and treatment strategies.

Published in Frontiers in Cellular and Infection Microbiology

ISSN: 2235-2988 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/Cellular-and-Infection-Microbiology

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