Parasites & Vectors (Dec 2012)

Acaricidal activity of ethanolic extract from aerial parts of <it>Tagetes patula</it> L. (Asteraceae) against larvae and engorged adult females of <it>Rhipicephalus sanguineus</it> (Latreille, 1806)

  • Politi Flávio Augusto Sanches,
  • Figueira Glyn Mara,
  • Araújo Andréa Mendez,
  • Sampieri Bruno Rodrigues,
  • Mathias Maria Izabel Camargo,
  • Szabó Matias Pablo Juan,
  • Bechara Gervásio Henrique,
  • dos Santos Lourdes Campaner,
  • Vilegas Wagner,
  • Pietro Rosemeire Cristina Linhari Rodrigues

DOI
https://doi.org/10.1186/1756-3305-5-295
Journal volume & issue
Vol. 5, no. 1
p. 295

Abstract

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Abstract Background The tick Rhipicephalus sanguineus is the species with the largest worldwide distribution and is proven to be involved in the transmission of pathogens such as Babesia canis, Ehrlichia canis, Coxiella burnetii, Rickettsia ricketsii, Rickettsia conorii, among others. Studies have demonstrated acquisition of resistance to some of the active principles used in commercial formulations of acaricides. Tagetes patula (Asteraceae) is a plant with highlighted economic and commercial importance due to the production of secondary metabolites with insecticide and acaricide potential, mainly flavonoids, thiophenes and terpenes. Methods The in vitro acaricide action of the ethanolic 70% extract from aerial parts of T. patula, obtained by percolation, was evaluated against larvae and engorged adult females of Rhipicephalus sanguineus by immersion test for 5 minutes. The chemical characterization of this extract was done by liquid chromatography coupled with mass spectrometry (LC-MS), using direct injection of sample. Results Despite T. patula not proving lethal to adults in any of the concentrations tested, at 50.0 mg/mL oviposition rate decreased by 21.5% and eliminated 99.78% of the larvae. Also it was determined that the best results were obtained with 5 minutes of immersion. From the chromatographic analysis twelve O-glycosylated flavonoids were identified. Conclusions This is the first report on the acaricidal activity of T. patula extract against Rh. sanguineus. If we consider the application of the product in the environment, we could completely eliminate the larval stage of development of the ixodid Rh. sanguineus.

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