Assembly and Analysis of the First Complete Mitochondrial Genome of Ficus carica Linn.

Abstract

Ficus carica Linn. is an important economic tree species with high developmental prospects and scientific research for edible and medicinal value. The F. carica chloroplast genome has recently been reported; however, the mitochondrial genome is still unexplored. We assembled the complete mitogenome of F. carica using reads from PacBio Biosciences sequencing platforms. The circular mitogenome F. carica has a length of 480,902 base pairs (bp), which contain 46 genes, including 27 protein-coding genes, 16 transfer RNA (tRNA) genes, and three ribosomal RNA (rRNA) genes. The base composition, codon usage, sequence repeats, RNA editing, and selective pressure were examined. We also conducted the phylogenetic analysis based on the mitogenomes of F. carica and 21 other taxa to know the evolutionary and taxonomic status of F. carica. Our analyses provided comprehensive information on the F. carica mitochondrial genome, which would facilitate evolutionary research in other fruit trees in the future.

Keywords

• ficus carica
• genome size
• phylogenetic analysis
• protein-coding gene
• repeats