A novel microfluidic flow-cytometry for counting numbers of single-cell β-actins

Lixing Liu; Beiyuan Fan; Hongyu Yang; Deyong Chen; Shuang Zhang; Junbo Wang; Jian Chen

Nanotechnology and Precision Engineering (Sep 2020)

A novel microfluidic flow-cytometry for counting numbers of single-cell β-actins

Lixing Liu,
Beiyuan Fan,
Hongyu Yang,
Deyong Chen,
Shuang Zhang,
Junbo Wang,
Jian Chen

Affiliations

Lixing Liu: State Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing 100190, China; School of Electronic, Electrical and Communication Engineering, University of Chinese Academy of Sciences, Beijing 100049, China
Beiyuan Fan: State Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing 100190, China; School of Electronic, Electrical and Communication Engineering, University of Chinese Academy of Sciences, Beijing 100049, China
Hongyu Yang: State Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing 100190, China; School of Future Technology, University of Chinese Academy of Sciences, Beijing 100049, China
Deyong Chen: State Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing 100190, China; School of Electronic, Electrical and Communication Engineering, University of Chinese Academy of Sciences, Beijing 100049, China; School of Future Technology, University of Chinese Academy of Sciences, Beijing 100049, China
Shuang Zhang: Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; Corresponding author.
Junbo Wang: State Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing 100190, China; School of Electronic, Electrical and Communication Engineering, University of Chinese Academy of Sciences, Beijing 100049, China; School of Future Technology, University of Chinese Academy of Sciences, Beijing 100049, China; Corresponding authors at: State Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing 100190, China.
Jian Chen: State Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing 100190, China; School of Electronic, Electrical and Communication Engineering, University of Chinese Academy of Sciences, Beijing 100049, China; School of Future Technology, University of Chinese Academy of Sciences, Beijing 100049, China; Corresponding authors at: State Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing 100190, China.

Journal volume & issue: Vol. 3, no. 3
pp. 156 – 161

Abstract

Read online

As a house keeping protein with stable expressions, β-actin is used as a loading control in normalization of western blotting. However, the actual numbers of β-actins at the single-cell level remain elusive. Based on a home-developed flow cytometry, single-cell numbers of β-actin from 8 cell types (subtypes) and 2 tumour patient samples were quantified as 9.62 ± 4.29 × 105 (A549, Ncell = 14,242), 6.46 ± 3.34 × 105 (Hep G2, Ncell = 35,932), 1.58 ± 0.90 × 106 (MCF 10A, Ncell = 16,650), 1.08 ± 0.48 × 106 (HeLa, Ncell = 26,151), 7.60 ± 4.34 × 105 (PC3, Ncell = 11,922), 1.10 ± 0.72 × 106 (SACC-83, Ncell = 13,616), 8.58 ± 4.54 × 105 (CAL 27, Ncell = 7271), 9.00 ± 4.69 × 105 (CAL 27-LN2, Ncell = 6222), 8.26 ± 4.48 × 105 (Oral Tumour Patient I, Ncell = 359), and 8.19 ± 5.12 × 105 (Oral Tumour Patient II, Ncell = 175), and were analyzed by statistical approaches including one-way analysis of variance, neural network based pattern recognition and Bayesian estimation, with varied expressions of β-actins among different cell types located. The dataset reported in this study may serve as a reference in future studies of quantitative protein analysis.

Published in Nanotechnology and Precision Engineering

ISSN: 1672-6030 (Print); 2589-5540 (Online)
Publisher: AIP Publishing LLC
Country of publisher: United States
LCC subjects: Technology: Engineering (General). Civil engineering (General)
Website: https://aip.scitation.org/journal/npe

About the journal

Abstract

Keywords