Genetic Diversity Analysis of Four Cultured Xenocypris davidi Populations Based on Mitochondrial D-loop Sequences

Shili LIU; Dawei CHEN; Jianbo ZHENG; Shun CHENG; Wenping JIANG; Meili CHI; Fengbo XIA; Fei LI

doi:10.16768/j.issn.1004-874X.2023.11.014

Guangdong nongye kexue (Nov 2023)

Genetic Diversity Analysis of Four Cultured Xenocypris davidi Populations Based on Mitochondrial D-loop Sequences

Shili LIU,
Dawei CHEN,
Jianbo ZHENG,
Shun CHENG,
Wenping JIANG,
Meili CHI,
Fengbo XIA,
Fei LI

Affiliations

Shili LIU: Zhejiang Institute of Freshwater Fisheries/Key Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture and Rural Affairs/Zhejiang Key Laboratory of Freshwater Aquatic Animal Genetic and Breeding, Huzhou 313001, China
Dawei CHEN: Zhejiang Institute of Freshwater Fisheries/Key Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture and Rural Affairs/Zhejiang Key Laboratory of Freshwater Aquatic Animal Genetic and Breeding, Huzhou 313001, China
Jianbo ZHENG: Zhejiang Institute of Freshwater Fisheries/Key Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture and Rural Affairs/Zhejiang Key Laboratory of Freshwater Aquatic Animal Genetic and Breeding, Huzhou 313001, China
Shun CHENG: Zhejiang Institute of Freshwater Fisheries/Key Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture and Rural Affairs/Zhejiang Key Laboratory of Freshwater Aquatic Animal Genetic and Breeding, Huzhou 313001, China
Wenping JIANG: Zhejiang Institute of Freshwater Fisheries/Key Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture and Rural Affairs/Zhejiang Key Laboratory of Freshwater Aquatic Animal Genetic and Breeding, Huzhou 313001, China
Meili CHI: Zhejiang Institute of Freshwater Fisheries/Key Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture and Rural Affairs/Zhejiang Key Laboratory of Freshwater Aquatic Animal Genetic and Breeding, Huzhou 313001, China
Fengbo XIA: College of Life Sciences, Huzhou University, Huzhou 313001, China
Fei LI: Zhejiang Institute of Freshwater Fisheries/Key Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture and Rural Affairs/Zhejiang Key Laboratory of Freshwater Aquatic Animal Genetic and Breeding, Huzhou 313001, China

DOI: https://doi.org/10.16768/j.issn.1004-874X.2023.11.014
Journal volume & issue: Vol. 50, no. 11
pp. 139 – 145

Abstract

Read online

【Objective】Xenocypris davidi is a freshwater economic fish, which feeds on humus, organic debris, plankton, and benthic animals, is one of the main fish for stock enhancement in Zhejiang Province. The research is aims to understand the impact of artificial breeding on the genetic diversity of X. davidi, and to provide basic data for the design and implementation of stock enhancement strategies for X. davidi in natural waters.【Method】D-loop region of mitochondrial DNA (mtDNA) of four cultured X. davidi populations from Changxing, Balidian, Shuangpu in Zhejiang Provice and Liling in Hunan Province were amplified and sequenced. The genetic diversity of the four populations was studied by sequence analysis.【Result】The mitochondrial D-loop sequence length of the X. davidi was 1 038-1 093 bp, and the base A+T content (65.3%) was significantly higher than the C+G content (34.7%), the average transitions and transversions ratio (TS/TV) was 4.6. A total of 101 mutation sites including 97 reduced information sites were detected in 128 D-loop sequences of X. davidi, and defining 19 haplotypes. Among them, the haplotype numbers of Changxing, Shuangpu, Balidian, and Liling populations were 5, 12, 4, and 2, respectively. The haplotype diversity (h) ranged from 0.226 to 0.915, and the nucleotide diversity (π) ranged from 0.00640 to 0.01433. The genetic distance between different breeding populations was 0.03782-0.88756, the genetic differentiation coefficient among populations was 0.78903 (P < 0.01), among which the degree of genetic difference between Changxing and Balidian populations was the lowest, and that of Shuangpu and Liling populations was the highest, and the genetic variation mainly occurred among populations.【Conclusion】There are certain differences in genetic diversity among the four cultured X. davidi populations. The results of the genetic variation and population structure of X. davidi will provide reference data for the comparative study on the genetic diversity of the X. davidi, and contribute to the resource protection.

Published in Guangdong nongye kexue

ISSN: 1004-874X (Print)
Publisher: Guangdong Academy of Agricultural Sciences
Country of publisher: China
LCC subjects: Agriculture
Website: http://gdnykx.cnjournals.org/gdnykx/ch/index.aspx

About the journal

Abstract

Keywords