Identifying the impact of G-Quadruplexes on Affymetrix 3′ Arrays using Cloud Computing

Memon Farhat N.; Owen Anne M.; Sanchez-Graillet Olivia; Upton Graham J.G.; Harrison Andrew P.

doi:10.1515/jib-2010-111

Journal of Integrative Bioinformatics (Jun 2010)

Identifying the impact of G-Quadruplexes on Affymetrix 3′ Arrays using Cloud Computing

Memon Farhat N.,
Owen Anne M.,
Sanchez-Graillet Olivia,
Upton Graham J.G.,
Harrison Andrew P.

Affiliations

Memon Farhat N.: Departments of Mathematical Sciences and Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex, CO4 3SQ, http://bioinformatics.essex.ac.uk/, United Kingdom of Great Britain and Northern Ireland
Owen Anne M.: Departments of Mathematical Sciences and Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex, CO4 3SQ, http://bioinformatics.essex.ac.uk/, United Kingdom of Great Britain and Northern Ireland
Sanchez-Graillet Olivia: Departments of Mathematical Sciences and Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex, CO4 3SQ, http://bioinformatics.essex.ac.uk/, United Kingdom of Great Britain and Northern Ireland
Upton Graham J.G.: Departments of Mathematical Sciences and Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex, CO4 3SQ,http://bioinformatics.essex.ac.uk/, United Kingdom of Great Britain and Northern Ireland
Harrison Andrew P.: Departments of Mathematical Sciences and Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex, CO4 3SQ, http://bioinformatics.essex.ac.uk/, United Kingdom of Great Britain and Northern Ireland

DOI: https://doi.org/10.1515/jib-2010-111
Journal volume & issue: Vol. 7, no. 2
pp. 12 – 20

Abstract

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A tetramer quadruplex structure is formed by four parallel strands of DNA/ RNA containing runs of guanine. These quadruplexes are able to form because guanine can Hoogsteen hydrogen bond to other guanines, and a tetrad of guanines can form a stable arrangement. Recently we have discovered that probes on Affymetrix GeneChips that contain runs of guanine do not measure gene expression reliably. We associate this finding with the likelihood that quadruplexes are forming on the surface of GeneChips.

Published in Journal of Integrative Bioinformatics

ISSN: 1613-4516 (Online)
Publisher: De Gruyter
Country of publisher: Germany
LCC subjects: Technology: Chemical technology: Biotechnology
Website: https://www.degruyter.com/view/j/jib

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