YES-10, A Combination of Extracts from <i>Clematis mandshurica</i> RUPR. and <i>Erigeron annuus</i> (L.) PERS., Prevents Ischemic Brain Injury in A Gerbil Model of Transient Forebrain Ischemia
Tae-Kyeong Lee,
Joon Ha Park,
Bora Kim,
Young Eun Park,
Jae-Chul Lee,
Ji Hyeon Ahn,
Cheol Woo Park,
Yoohun Noh,
Ji-Won Lee,
Sung-Su Kim,
Jong Dai Kim,
Moo-Ho Won
Affiliations
Tae-Kyeong Lee
Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Korea
Joon Ha Park
Department of Anatomy, College of Korean Medicine, Dongguk University, Gyeongju, Gyeongbuk 38066, Korea
Bora Kim
Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Korea
Young Eun Park
Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Korea
Jae-Chul Lee
Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Korea
Ji Hyeon Ahn
Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chuncheon, Gangwon 24252, Korea
Cheol Woo Park
Leefarm Co., Ltd, Hongcheon, Gangwon 25117, Korea
Yoohun Noh
Famenity Company, Gwacheon, Geyonggi 13837, Korea
Ji-Won Lee
Famenity Company, Gwacheon, Geyonggi 13837, Korea
Sung-Su Kim
Famenity Company, Gwacheon, Geyonggi 13837, Korea
Jong Dai Kim
Division of Food Biotechnology, School of Biotechnology, Kangwon National University, Chuncheon, Gangwon 24341, Korea
Moo-Ho Won
Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Korea
Clematis mandshurica RUPR. (CMR) and Erigeron annuus (L.) PERS. (EALP) have pharmacological effects including anti-inflammatory activity and been used in traditional medicines in Asia. However, neuroprotective effects of CMR and/or EALP extracts against brain ischemic insults have never been addressed. Thus, the aim of this study was to examine neuroprotective effects of YES-10, a combination of extracts from CMR and EALP (combination ratio, 1:1), in the hippocampus following ischemia/reperfusion in gerbils. Protection of neurons was investigated by cresyl violet staining, fluoro-jade B histofluorescence staining and immunohistochemistry for neuronal nuclei. In addition, attenuation of gliosis was studied by immunohistochemistry for astrocytic and microglial markers. Treatments with 50 or 100 mg/kg YES-10 failed to protect neurons in the hippocampus after ischemia/reperfusion injury. However, administration of 200 mg/kg YES-10 protected neurons from ischemia/reperfusion injury and attenuated reactive gliosis. These findings strongly suggest that a combination of extracts from CMR and EALP can be used as a prevention approach/drug against brain ischemic damage.
