Structural and electronic determinants of lytic polysaccharide monooxygenase reactivity on polysaccharide substrates

T. J. Simmons; K. E. H. Frandsen; L. Ciano; T. Tryfona; N. Lenfant; J. C. Poulsen; L. F. L. Wilson; T. Tandrup; M. Tovborg; K. Schnorr; K. S. Johansen; B. Henrissat; P. H. Walton; L Lo Leggio; P. Dupree

doi:10.1038/s41467-017-01247-3

Nature Communications (Oct 2017)

Structural and electronic determinants of lytic polysaccharide monooxygenase reactivity on polysaccharide substrates

T. J. Simmons,
K. E. H. Frandsen,
L. Ciano,
T. Tryfona,
N. Lenfant,
J. C. Poulsen,
L. F. L. Wilson,
T. Tandrup,
M. Tovborg,
K. Schnorr,
K. S. Johansen,
B. Henrissat,
P. H. Walton,
L Lo Leggio,
P. Dupree

Affiliations

T. J. Simmons: Department of Biochemistry, University of Cambridge
K. E. H. Frandsen: INRA, UMR 1163 BBF (Biodiversité et Biotechnologie Fongiques)
L. Ciano: Department of Chemistry, University of York
T. Tryfona: Department of Biochemistry, University of Cambridge
N. Lenfant: Architecture et Fonction des Macromolécules Biologiques (AFMB), CNRS, Aix-Marseille Université
J. C. Poulsen: Department of Chemistry, University of Copenhagen
L. F. L. Wilson: Department of Biochemistry, University of Cambridge
T. Tandrup: Department of Chemistry, University of Copenhagen
M. Tovborg: Research and Development, Novozymes A/S
K. Schnorr: Research and Development, Novozymes A/S
K. S. Johansen: Department of Geoscience and Natural Resources Management, Copenhagen University
B. Henrissat: Architecture et Fonction des Macromolécules Biologiques (AFMB), CNRS, Aix-Marseille Université
P. H. Walton: Department of Chemistry, University of York
L Lo Leggio: Department of Chemistry, University of Copenhagen
P. Dupree: Department of Biochemistry, University of Cambridge

DOI: https://doi.org/10.1038/s41467-017-01247-3
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 12

Abstract

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Copper-dependent lytic polysaccharide monooxygenases (LPMOs) oxidatively cleave polysaccharides. Here the authors present a structure-function characterization of fungal LPMOs, showing that a particular LPMO cleaves xylan using a mechanism that involves an alternative copper coordination geometry.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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